Effects of Recloning on the Efficiency of Production of α1,3-Galactosyltransferase Knockout Pigs
スポンサーリンク
概要
- 論文の詳細を見る
Obtaining sufficient transgenic cells via selective cultivation of genetically manipulated somatic cells is difficult due to the limited number of cell divisions. Additionally, if irreversible mutations in a cells chromosomes occur during selective cultivation and the cell is used as the nuclear donor, somatic cell nuclear transfer (SCNT) embryos often exhibit abnormal development. On the other hand, a SCNT method in which fetal cells derived from SCNT embryos are used as the nuclear donor (recloning method) is an effective technique for obtaining large quantities of transgenic cells. In this study, we compared the in vivo development rate of SCNT embryos produced from porcine α1-3 galactosyltransferase gene knockout (GTKO) cells by a recloning method with that of SCNT embryos produced without recloning from porcine GTKO cells (direct method). In the direct method, 557 and 462 cloned embryos were produced using two types of activation methods, the two-step activation (TA) method and the delayed activation (DA) method, and then transferred into 6 and 4 recipients, respectively, but no piglets were born from these recipients. In the recloning method, 956 and 1038 cloned embryos were produced using the TA and DA methods, respectively, and then transferred to 8 and 7 recipients, respectively. Two piglets were born from one recipient in the TA group and 6 piglets were born from 3 recipients in the DA group. This report indicates that the recloning method improved the developmental capacity of SCNT embryos reconstructed with gene-targeted somatic cells.
- 日本繁殖生物学会の論文
- 2008-02-01
著者
-
村上 浩
Earth Observation Research Center, Japan Aerospace Exploration Agency
-
NAGASHIMA Hiroshi
Laboratory of Developmental Engineering, Department of Life Sciences, School of Agriculture, Meiji U
-
KUROME Mayuko
Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji Un
-
FUJIMURA Tatsuya
The Animal Engineering Research Institute Co., Ltd
-
TAKAHAGI Yoichi
The Animal Engineering Research Institute Co., Ltd
-
MURAKAMI Hiroshi
The Animal Engineering Research Institute Co., Ltd
-
Takahagi Y
The Animal Engineering Research Institute
-
Fujimura Tatsuya
The Animal Engineering Research Institute
-
Nagashima Hiroshi
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
-
Murakami H
Earth Observation Research Center Japan Aerospace Exploration Agency
-
Kurome Mayuko
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
-
SHIGEHISA Tamotsu
The Animal Engineering Research Institute
-
Nagashima Hiroshi
Lab. Of Developmental Engineering Dep. Of Life Sciences School Of Agriculture Meiji Univ.
-
Nagashima Hiroshi
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
-
Kurome Mayuko
Laboratory Of Developmental Engineering Department Of Life Science School Of Agriculture Meiji Unive
-
Nagashima Hiroshi
Laboratory For Evolutionary Morphology Riken Cdb
-
Kurose Mayuko
Laboratory of Developmental Engineering, Department of Life Science, School of Agriculture, Meiji University
-
TAKAHAGI Yoichi
The Animal Engineering Research Institute
関連論文
- ADEOS-II/GLIプロジェクトの概要とハイライト
- Validation of ADEOS-II GLI Ocean Color Products Using In-Situ Observations
- 卵細胞質内注入法を用いた牛の核移植
- Developmental Ability of Porcine In Vitro Matured Oocytes at the Meiosis II Stage After Vitrification
- GLI海洋プロダクトの開発と利用研究(宇宙応用シンポジウム : ADEOS-II)
- 日本周辺の海洋観測ブイにおける1日から1年の周期の海面水温と海面熱フラックスの関係
- Production of Cloned Pigs by Nuclear Transfer of Preadipocytes Following Cell Cycle Synchronization by Differentiation Induction
- 人工衛星による地球規模の海洋基礎生産推定 (特集 海色リモートセンシングと海洋基礎生産力)
- Cloned Porcine Embryos can Maintain Developmental Ability after Cryopreservation at the Morula Stage
- Production Efficiency and Telomere Length of the Cloned Pigs Following Serial Somatic Cell Nuclear Transfer
- ヒト血清アルブミンとEGFPを発現する形質転換ブタの開発
- 電気的活性化後のブタ体外成熟卵の発生能力
- ADEOS/OCTS海色データにおける雲近傍での色素濃度推定の異常と識別手法
- Evaluation of ADEOS-II GLI Ocean Color Atmospheric Correction Using SIMBADA Handheld Radiometer Data
- IgE-antibody Specificities of the Patients Allergic to Meat Products
- IgE-antibody Specificities of the Patients Allergic to Meat Products
- Algorithm and Validation of Sea Surface Temperature Observation Using MODIS Sensors aboard Terra and Aqua in the Western North Pacific
- Difference Characteristics of Sea Surface Temperature Observed by GLI and AMSR Aboard ADEOS-II
- Sea Surface Temperature Observation by Global Imager (GLI)/ADEOS-II : Algorithm and Accuracy of the Product
- The Sea Surface Temperature Product Algorithm of the Ocean Color and Temperature Scanner (OCTS) and Its Accuracy
- Calibration and Validation of the Ocean Color Version-3 Product from ADEOS OCTS
- Calcium Release at Fertilization : Artificially Mimicking the Oocyte's Response to Sperm
- Retrieval of Chlorophyll-a Concentration via Linear Combination of ADEOS-II Global Imager Data
- Molecular cloning of a pig homologue of membrane cofactor protein (CD46)
- ブタ卵の成熟条件がMII期紡錘体の形態に及ぼす影響
- Production of Transgenic and Non-Transgenic Clones in Miniature Pigs by Somatic Cell Nuclear Transfer
- Production of Cloned Pigs by Nuclear Transfer of Preadipocytes Following Cell Cycle Synchronization by Differentiation Induction
- Effects of Sperm Pretreatment on Efficiency of ICSI-Mediated Gene Transfer in Pigs
- Association Between Embryonic Loss and Damage to the Zona Pellucida by Invasive Micromanipulation During Oviductal Transfer of Early-Stage Embryos in Pigs
- Effects of Maturation Conditions on Spindle Morphology in Porcine MII Oocytes
- In Vitro and in Vivo Anti-platelet Effects of Enzymatic Hydrolysates of Collagen and Collagen-related Peptides
- Effects of Recloning on the Efficiency of Production of α1,3-Galactosyltransferase Knockout Pigs
- Production of a Transgenic Pig Expressing Human Albumin and Enhanced Green Fluorescent Protein
- Autopodial Development in the Sea Turtles Chelonia mydas and Caretta caretta
- Application of Genetically Modified and Cloned Pigs in Translational Research
- In Vitro Development and Postvitrification Survival of Cloned Feline Embryos Derived from Preadipocytes
- Development and Perspectives of Perfusion Treatment for Liver Failure
- Cloned Porcine Embryos can Maintain Developmental Ability after Cryopreservation at the Morula Stage
- Biochemical Effects of High Hydrostatic Pressure on the Lysosome and Proteases Involved in It
- Effect of High Pressure on the Protease Activities in Meat(Food & Nutrition)
- What Triggered Turtle Shell Evolution? : Identification of Gene Co-Option in Carapace Formation(Organogenesis and Evolution,Symposium,PROCEEDING OF THE 75^ ANNUAL MEETING OF THE ZOOLOGICAL SOCIETY OF JAPAN)
- Atmospheric Correction Scheme for GLI with Absorptive Aerosol Correction
- The Production of a Diabetic Mouse Using Constructs Encoding Porcine Insulin Promoter-Driven Mutant Human Hepatocyte Nuclear Factor-1α
- Hollow Fiber Vitrification: A Novel Method for Vitrifying Multiple Embryos in a Single Device
- Hollow Fiber Vitrification : A Novel Method for Vitrifying Multiple Embryos in a Single Device
- Production of Diabetic Offspring Using Cryopreserved Epididymal Sperm by In Vitro Fertilization and Intrafallopian Insemination Techniques in Transgenic Pigs
- In Vitro Development of Porcine Nuclear Transfer Embryos Reconstructed by Intracytoplasmic Microinjection of Cumulus Cell Nuclei into In Vitro Matured Oocytes.
- Evaluation of the Quality of Porcine Somatic Cell Nuclear Transfer Embryo by Gene Transcription Profiles
- The Production of a Diabetic Mouse Using Constructs Encoding Porcine Insulin Promoter-Driven Mutant Human Hepatocyte Nuclear Factor-1.ALPHA.
- Production of Transgenic-cloned Pigs Expressing Far-red Fluorescent Protein, Monomeric Plum