Synthesis of a New Cre Recombinase Gene Based on Optimal Codon Usage for Mammalian Systems.

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概要

• 論文の詳細を見る

• The origin of the Cre recombinase gene is bacteriophage P1, and thus the codon usages are different from in mammals. In order to adapt this codon usage for mammals, we synthesized a "mammalian Cre recombinase gene" and examined its expression in Chinese hamster ovarian tumor (CHO) cells. Significant increases in protein production as well as mRNA levels were observed. When the recombination efficiency was compared using CHO cell transfectants having a cDNA containing loxP sites, the "mammalian Cre recombinase gene" recombined the loxP sites much more efficiently than the wild-type Cre recombinase gene.

• 社団法人 日本生化学会の論文

著者

• SHIRAKURA Ryota

  Division of Organ Transplantation, Department of Regenerative Medicine, Osaka University Graduate Sc

• YAMADA Mako

  Division of Organ Transplantation, Osaka University Graduate School of Medicine

• Koresawa Yukie

  Division Of Organ Transplantation Osaka University Graduate School Of Medicine

• Matsunami Katsuyoshi

  Division Of Organ Transplantation Biomedical Research Center Osaka University Graduate School Of Med

• MIYAGAWA Shuji

  Division of Organ Transplantation (E9), Department of Surgery, Osaka University Graduate School of Medicine

• Matsunami Katsuyoshi

  Division of Organ Transplantation, Biomedical Research Center Osaka University Graduate School of Medicine

• Ikawa Masahito

  Genorne Information Research Center, Osaka University

• Okabe Masaru

  Genorne Information Research Center, Osaka University

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