Evidence for Existence of cAMP-Epac Signaling in the Heads of Mouse Epididymal Spermatozoa

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概要

• 論文の詳細を見る

• Cyclic adenosine 3,5-monophosphate (cAMP) signaling regulates the expression of fertilizing ability in mammalian spermatozoa. Many articles indicate that this signaling is mediated mainly via protein kinase A. Recently, a guanine nucleotide exchange factor for small G protein Rap1 (an exchange protein directly activated by cAMP: Epac) was discovered as a new mediator of cAMP signaling in somatic cells. The aim of this study was to reveal the existence of cAMP-Epac signaling in mouse spermatozoa. Northern blot analysis and in situ hybridization suggested that Epac1 and Epac 2 mRNAs were transcribed in the seminiferous epithelia of the testis. This shows that expression of Epac mRNAs is present in mouse testicular germ cells. Indirect immunofluorescence with specific polyclonal antibodies suggested possible co-localization of Epac1 and Rap1 proteins in the heads of epididymal spermatozoa. Moreover, treatment of epididymal spermatozoa with an Epac-specific cAMP analog, 8-pMeOPT-2-O-Me-cAMP, induced activation of Rap1, as revealed with a commercial kit for pull-down assay. These results indicate the existence of cAMP-Epac signaling in the heads of mouse epididymal spermatozoa.

• 日本繁殖生物学会の論文

著者

• LEE Jibak

  Graduate School of Science and Technology, Kobe University

• HARAYAMA Hiroshi

  Graduate School of Science and Technology, Kobe University

• Lee Jibak

  Graduate School Of Science And Technology Kobe University

• Harayama Hiroshi

  Graduate School Of Agricultural Sci. Kobe Univ.

• GOTO Namiko

  Graduate School of Science and Technology and Graduate School of Agricultural Science, Kobe Universi

• Amano Reiko

  Graduate School Of Science And Technology Kobe University

• Goto Namiko

  Graduate School Of Science And Technology And Graduate School Of Agricultural Science Kobe Universit

• GOTO Namiko

  Graduate School of Science and Technology, Kobe University

関連論文

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• Detection of 25-kDa Anti-Agglutinin in Epididymal Plasma and Spermatozoa Collected from Various Regions of Boar Epididymis
• Evidence for Existence of cAMP-Epac Signaling in the Heads of Mouse Epididymal Spermatozoa
• Evidence for Existence of cAMP-Epac Signaling in the Heads of Mouse Epididymal Spermatozoa
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• Premature Capacitation of Frozen-Thawed Spermatozoa from Subfertile Japanese Black Cattle
• Motility and Penetrability into Zona-Free Hamster Eggs of Boar Spermatozoa Collected from Various Regions of the Epididymis
• Effect of H89 on the meiotic resumption of pig oocytes

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