Turnover of Cell Wall Polysaccharides during the Cell Cycle in a Synchronous Culture of Catharanthus roseus
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概要
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Pulse-chase experiments were done using a synchronous culture of Catharanthus roseus in order to study cell wall turnover during the cell cycle. [^<14>C]Glucose was fed for l h to cells 35 and 49 h after the restart of the cell cycle. Radioactivity was then diluted with a large amount of cold glucose and chased during the early G1 phase after the first cell division, the time at which an increase in the amount of cell walls mainly took place. A pulse-chase with [^<14>C]glucose was also made during the S phase when cell walls had not increased so much. Radioactivity of the EDTA-soluble (pectin) fraction decreased during the chase in the early G1 phase; whereas, the radioactivities of the other cell wall fractions, as well as extracellular polysaccharide (ECP) increased during the chase, both in the early G1 and in the S phases. The radioactivity of uronic acid in ECP was higher in the early G1 phase than in the S phase. These results indicate that an active turnover of pectin may take place in the early G1 phase after the first cell division.
- 日本植物生理学会の論文
著者
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Komamine Atsushi
Department Of Botany Faculty Of Science The University Of Tokyo:(present)biological Institute Facult
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Komamine Atsushi
Department Of Botany Faculty Of Science University Of Tokyo:(present)biological Institute Tohoku Uni
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Amino Shin-ichi
Department Of Botany Faculty Of Science University Of Tokyo:(present)biological Institute Tohoku Uni
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Amino Shin-ichi
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
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