Relationship between Tracheary Element Differentiation and DNA Synthesis in Single Cells Isolated from the Mesophyll of Zinnia elegans : Analysis by Inhibitors of DNA Synthesis

スポンサーリンク

概要

• 論文の詳細を見る

• Effects of inhibitors of DNA synthesis on tracheary element (TE) differentiation were investigated in a culture of single cells isolated from the mesophyll of Zinnia elegans L. cv. Canary bird. In this system, neither mitosis nor replication of the whole genome during the S phase in the cell cycle is a prerequisite for TE differentiation [Fukuda and Komamine (1980) Plant Physiol. 65: 61, unpublished data]. Fluorouracil (FU), fluorodeoxyuridine (FUdR), mitomycin C (MC), arabinosyl cytosine (ara-C) and aphidicolin, inhibitors of DNA synthesis, prevented the incorporation of [3H]-thymidine into nucleic acid, cell division and cytodifferentiation to TE. However, neither FUdR nor aphidicolin prevented the incorporation of [^<14>C]-leucine into protein. Thymidine reversed the inhibitory effect of FUdR when given simultaneously with FUdR. These results show that the inhibitors of DNA synthesis prevent TE differentiation via blockage of the synthesis of some DNA, although replication of the whole genome during the S phase is not a prerequisite for cytodifferentiation. The role of DNA synthesis in TE differentiation is discussed.

• 日本植物生理学会の論文

著者

• Komamine Atsushi

  Department Of Botany Faculty Of Science The University Of Tokyo:(present)biological Institute Facult

• Fukuda Hiroo

  Deparment Of Biological Sciences Graduate School Of Science The University Of Tokyo

• Fukuda Hiroo

  Department Of Botany Faculty Of Science University Of Tokyo

関連論文

• Gene Trapping in Arabidopsis Reveals Genes Involved in Vascular Development
• Vascular cell expression patterns of Arabidopsis bZIP group I genes
• DISSECTION OF ARABIDOPSIS RAB GTPases IN ASSOCIATION WITH VASCULAR FORMATION
• FUNCTIONAL ANALYSIS OF NAC-DOMAIN PROTEINS THAT REGULATE XYLEM VESSEL DIFFERENTIATION
• Cross talk between auxin and other hormones in the early process of transdifferentiation into tracheary elements
• Cell biological analysis of the van3 mutant of Arabidopsis involved in vascular patterning
• Isolation and Characterization of a Novel Peroxidase Gene ZPO-C Whose Expression and Function are Closely Associated with Lignification during Tracheary Element Differentiation
• Co-Regulation of Brassinosteroid Biosynthesis-Related Genes During Xylem Cell Differentiation
• Analysis of xylogen homologous genes in Arabidopsis
• A Possible Role of Glutathione and Glutathione Disulfide in Tracheary Element Differentiation in the Cultured Mesophyll Cells of Zinnia elegans
• Autolysis during In Vitro Tracheary Element Differentiation: Formation and Location of the Perforation
• Microarray analysis of compensated cell enlargement in angustifolia3
• Class III Homeodomain Leucine-Zipper Proteins Regulate Xylem Cell Differentiation
• Establishment of a Sensitized Immunoblotting Method for Measuring Plant Tubulin Content
• Regulation of Tubulin Degradation in Isolated Zinnia Mesophyll Cells in Culture
• Identification and molecular characterization of novel anther-specific genes in Oryza sativa L. by using cDNA microarray
• Inhibition of Transdifferentiation into Tracheary Elements by Polar Auxin Transport Inhibitors Through Intracellular Auxin Depletion
• Involvement of Extracellular Dilignols in Lignification During Tracheary Element Differentiation of Isolated Zinnia Mesophyll Cells
• Transfer of phenylpropanoids via the medium between xylem cells in Zinnia xylogenic culture
• Inhibition of Cell Division and DNA Synthesis by Gibberellin in Isolated Zinnia Mesophyll Cells
• Studies of the Plant Cell Cycle in Synchronous Cultures of Catharanthus roseus Cells : GROWTH AND DEVELTOPMENT
• Programming of Cell Death during Xylogenesis
• Spatial and Temporal Tracing of Vessel Differentiation in Young Arabidopsis Seedlings by the Expression of an Immature Tracheary Element-specific Promoter
• Promotion of Transcript Accumulation of Novel Zinnia Immature Xylem-Specific HD-Zip III Homeobox Genes by Brassinosteroids
• Analysis of Early Processes in Wound-Induced Vascular Regeneration using TED3 and ZeHB3 as Molecular Markers
• Primary Phloem-Specific Expression of a Zinnia elegans Homeobox Gene
• An Arabinogalactan Protein(s) is a Key Component of a Fraction that Mediates Local Intercellular Communication Involved in Tracheary Element Differentiation of Zinnia Mesophyll Cells
• Frequency Improvement of Somatic Embryogenesis at High Embryo Density by Partial Replacement of Medium in Carrot Suspension Cultures
• Changes in Activities of Enzymes Involved in General Phenylpropanoid Metabolism during the Induction and Reduction of Anthocyanin Synthesis in a Carrot Suspension Cultue as Regulated by 2,4-D
• Establishment of a new Zinnia experimental system including separation of the distinct cell population with flow cytometry and its culture
• Preferential and Asymmetrical Accumulation of a Rac Small GTPase mRNA in Differentiating Xylem Cells of Zinnia elegans
• The mechanism of changes in respiratory activity during callus formation in carrot root slices cultured in vitro
• The Use of Multiple Transcription Starts Causes the Dual Targeting of Arabidopsis Putative Monodehydroascorbate Reductase to Both Mitochondria and Chloroplasts
• Plant meristems : CLAVATA3/ESR-related signaling in the shoot apical meristem and the root apical meristem
• Cell differentiation in the longitudinal veins and formation of commissural veins in rice (Oryza sativa) and maize (Zea mays)
• A New Endopeptidase Activity Induced in the Last Stage of Tracheary Element Differentiation of Zinnia Cells
• The Receptor-Like Kinase SOL2 Mediates CLE Signaling in Arabidopsis
• Identification of intercellullar communication factors in Arabidopsis xylogenic cell suspension culture
• Development of Sink Capacity of the "Storage Root" in a Radish Variety with a Low Ratio of "Storage Root" to Shoot
• Development of Sink Capacity of the "Storage Root" in a Radish Cultivar with a High Ratio of "Storage Root" to Shoot
• Effects of Growth Regulators on the Induction of Anthocyanin Synthesis in Carrot Suspension Cultures
• Effects of Nutrient Limitation and _γ-Irradiation on Tracheary Element Differentiation and Cell Division in Single Mesophyll Cells of Zinnia elegans
• Changes in the Activities of Various Glycosidases during Carrot Cell Elongation in a 2,4-D-Free Medium
• Molecular genetic analysis of a phloem specific transcription factor, Zinnia ZeHB3, and its Arabidopsis homolog, AtHB5
• Glucans in the Cell Walls Regenerated from Vinca rosea Protoplasts
• Effects of Culture Conditions on Cell Division and Composition of Regenerated Cell Walls in Vinca rosea Protoplasts
• Tissue-Specific Localization of mRNA for Carrot Homeobox Genes, CHBs, in Carrot Somatic Embryos
• Relationship between Tracheary Element Differentiation and DNA Synthesis in Single Cells Isolated from the Mesophyll of Zinnia elegans : Analysis by Inhibitors of DNA Synthesis
• A Rapid Method for Transformation of Carrot (Daucus carota L.) by Using Direct Somatic Embryogenesis
• Changes in the Synthesis of RNA and Protein during Tracheary Element Differentiation in Single Cells Isolated from the Mesophyll of Zinnia elegans
• A Change in Tubulin Synthesis in the Process of Tracheary Element Differentiation and Cell Division of Isolated Zinnia Mesophyll Cells
• Analysis by in situ Hybridization of the Expression of Elongation Factor 1α in the Carrot Cells during Somatic Embryogenesis
• Vessel element formation in cultured carrot-root phloem slices
• RESPIRATORY METABOLISM IN THE PHLOEM, XYLEM AND CAMBIUM OF CARROT ROOT
• Turnover of Cell Wall Polysaccharides during the Cell Cycle in a Synchronous Culture of Catharanthus roseus
• HD-Zip III Homeobox Genes that Include a Novel Member, ZeHB-13 (Zinnia)/ATHB-15 (Arabidopsis), are Involved in Procambium and Xylem Cell Differentiation
• ANGUSTIFOLIA3 Plays Roles in Adaxial/Abaxial Patterning and Growth in Leaf Morphogenesis
• Mitogen-Activated Protein Kinase Regulated by the CLAVATA Receptors Contributes to Shoot Apical Meristem Homeostasis
• Diverse Functions of Plant Peptides : Entering a New Phase
• A Novel Function of TDIF-Related Peptides : Promotion of Axillary Bud Formation
• CLE Peptides can Negatively Regulate Protoxylem Vessel Formation via Cytokinin Signaling
• The CKH1/EER4 Gene Encoding a TAF12-Like Protein Negatively Regulates Cytokinin Sensitivity in Arabidopsis thaliana
• The CKH2/PKL Chromatin Remodeling Factor Negatively Regulates Cytokinin Responses in Arabidopsis Calli
• Expression and Genome-Wide Analysis of the Xylogen-Type Gene Family

もっと見る 閉じる

スポンサーリンク