Characterization of the Plastidic Phosphate Translocators in the Inducible Crassulacean Acid Metabolism Plant Mesembryanthemum crystallinum

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概要

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• In plant Mesembryanthemum crystallinum, which has the inducible crassulacean acid metabolism (CAM), isoforms of plastidic phosphate translocators (pPTs) are categorized into three subfamilies: the triose phosphate/phosphate translocator (McTPT1), the phosphoenolpyruvate/phosphate translocator (McPPT1), and the glucose 6-phosphate/phosphate translocator (McGPT1 and McGPT2). In order to elucidate the physiological roles of these pPTs in M. crystallinum, we determined the substrate specificity of each pPT isoform. The substrate specificities of McTPT1, McPPT1, and McGPT1 showed overall similarities to those of orthologs that have been characterized. In contrast, for glucose 6-phosphate, McGPT2 showed higher selectivity than McGPT1 and other GPT orthologs. Because the expression of McGTP2 is specific to CAM while that of McGTP1 is constitutively expressed in both the C3- and the CAM-state in M. crystallinum, we propose that McGPT2 functions as a CAM system-specific GPT in this plant.

著者

• Tozawa Yuzuru

  Cell-free Sci. And Technol. Res. Center And The Venture Business Lab. Ehime Univ.

• Nozawa Akira

  Cell-free Science And Technology Research Center And The Venture Business Laboratory Ehime Universit

• Ohnishi Jun-ichi

  Department Of Biochemistry And Molecular Biology Faculty Of Science Saitama University

• NISHIYAMA Yoshitaka

  Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, and Institute for Environmental Science and Technology, Saitama University

• OKADA Yusuke

  Cell-Free Science and Technology Research Center and The Venture Business Laboratory, Ehime University

• AZAD Muhammad

  Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, and Institute for Environmental Science and Technology, Saitama University

• OHNISHI Jun-ichi

  Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, and Institute for Environmental Science and Technology, Saitama University

• KORE-EDA Shin

  Comprehensive Analysis Center for Science, Saitama University

• TAKASHI Kazuki

  Department of Biochemistry and Molecular Biology, Graduate School of Science and Engineering, and Institute for Environmental Science and Technology, Saitama University

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