Expression of Parsley Flavone Synthase I Establishes the Flavone Biosynthetic Pathway in Arabidopsis thaliana
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概要
- 論文の詳細を見る
Arabidopsis thaliana lacks the flavone biosynthetic pathway, probably because of a lack or low activity of a flavone synthase. To establish this biosynthetic pathway in Arabidopsis, we subjected this model plant to transformation with the parsley gene for flavone synthase type I (FNS-I). Transgenic seedlings expressing FNS-I were cultured in liquid medium with or without naringenin, and plant extracts were then analyzed by high-performance liquid chromatography. In contrast to wild-type seedlings, the transgenic seedlings accumulated substantial amounts of apigenin, which is produced from naringenin by FNS-I, and the apigenin level correlated with the abundance of FNS-I mRNA in three different transgenic lines. These results indicate that the FNS-I transgene produces a functional enzyme that catalyzes the conversion of naringenin to apigenin in Arabidopsis. These FNS-I transgenic lines should prove useful in investigating the in vivo functions of enzymes that mediate the synthesis of the wide variety of flavones found in other plants.
- 社団法人 日本農芸化学会の論文
- 2008-04-23
著者
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TOZAWA Yuzuru
Cell-Free Science and Technology Research Center, Ehime University
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Tozawa Yuzuru
Cell-free Sci. And Technol. Res. Center And The Venture Business Lab. Ehime Univ.
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NOZAWA Akira
Cell-Free Science and Technology Research Center, and The Venture Business Laboratory, Ehime Univers
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Yamamoto Tomio
Cell-free Science And Technology Research Center And The Venture Business Laboratory Ehime Universit
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YUN Choong-Soo
Cell-Free Science and Technology Research Center and the Venture Business Laboratory, Ehime Universi
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Nozawa Akira
Cell-free Science And Technology Research Center And The Venture Business Laboratory Ehime Universit
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Tozawa Yuzuru
Cell-free Science And Technology Research Center And The Venture Business Laboratory Ehime Universit
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Yun Choong-soo
Cell-free Sci. And Technol. Res. Center And The Venture Business Lab. Ehime Univ.
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