Construction of a Protein Library of Arabidopsis Transcription Factors Using a Wheat Cell-Free Protein Production System and Its Application for DNA Binding Analysis
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概要
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We created a protein library consisting of 647 Arabidopsis transcription factors (TFs) using a wheat cell-free system. The quality of proteins in the library was checked by binding assay of bZIP family proteins. Screening of TFs binding to 5′-regulatory regions of FLC and LFY was conducted using the library, and MYB67 and GBF1 were found to be binding factors.
- 社団法人 日本農芸化学会の論文
著者
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SEKI Motoaki
Plant Functional Genomics Research Group, RIKEN Genomic Sciences Center
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SHINOZAKI Kazuo
Gene Discovery Research Team, RIKEN Plant Science Center (PSC), RIKEN Tsukuba Institute
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NOZAWA Akira
Cell-Free Science and Technology Research Center, and The Venture Business Laboratory, Ehime Univers
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ENDO Yaeta
Cell-Free Science and Technology Research Center, and The Venture Business Laboratory, Ehime Univers
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MATSUBARA Yuko
Cell-Free Science and Technology Research Center, and Venture Business Laboratory, Ehime University
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TANAKA Yoshinori
Cell-Free Science and Technology Research Center, and Venture Business Laboratory, Ehime University
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TAKAHASHI Hirotaka
Cell-Free Science and Technology Research Center, and Venture Business Laboratory, Ehime University
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AKAGI Tatsuya
Cell-Free Science and Technology Research Center, and Venture Business Laboratory, Ehime University
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SAWASAKI Tatsuya
Cell-Free Science and Technology Research Center, and Venture Business Laboratory, Ehime University
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