In Vitro Protein Import of a Putative Amino Acid Transporter from Arabidopsis thaliana into Chloroplasts and Its Suborganellar Localization
スポンサーリンク
概要
- 論文の詳細を見る
We identified a gene product of At5g19500 (At5g19500p) from Arabidopsis thaliana that is homologous to EcTyrP, a tyrosine-specific transporter from Escherichia coli. Computational analyses of the amino acid sequence of At5g19500p predicted 11 transmembrane domains (TMDs) and a potential plastid targeting signal at its amino terminus. As a first step toward understanding the possible role of At5g19500p in plant cells, we attempted to determine the localization of At5g19500p by an in vitro chloroplastic import assay using At5g19500p translated in a cell-free wheat germ system (Madin et al., Proc. Natl. Acad. Sci. USA, 97, 559–564 (2000)), followed by subfractionation of the chloroplasts. At5g19500p was successfully imported into chloroplasts, and the newly transported mature form of At5g19500p was recovered from the inner envelope membrane.
- 2011-11-23
著者
-
TOZAWA Yuzuru
Cell-Free Science and Technology Research Center, Ehime University
-
Tozawa Yuzuru
Cell-free Science And Technology Research Center Ehime University
-
Tozawa Yuzuru
Cell-free Sci. And Technol. Res. Center And The Venture Business Lab. Ehime Univ.
-
Nozawa Akira
Cell-free Science And Technology Research Center And The Venture Business Laboratory Ehime Universit
-
Akita Mitsuru
The United Graduate School Of Agricultural Sciences Ehime Univ.
-
SATTASUK Kwanchanok
The United Graduate School of Agricultural Sciences, Ehime University
-
Kakinuma Yoshimi
The United Graduate School Of Agricultural Sciences Ehime University
-
Sattasuk Kwanchanok
The United Graduate School Of Agricultural Sciences Ehime University
-
Nozawa Akira
Cell-free Science And Technology Research Center Ehime University
関連論文
- Characterization of a Rice Nuclear-Encoded Plastid RNA Polymerase Gene OsRpoTp
- Differential Expression of Three Plastidial Sigma Factors, OsSIG1, OsSIG2A, and OsSIG2B, during Leaf Development in Rice
- Expression Profiling of Translation-associated Genes in Sporulating Bacillus subtilis and Consequence of Sporulation by Gene Inactivation(Microbiology & Fermentation Technology)
- OsRALyase1, a Putative F-Box Protein Identified in Rice, Oryza sativa, with Enzyme Activity Identical to That of Wheat RALyase(Biochemistry & Molecular Biology)
- A Cell-Free Translation and Proteoliposome Reconstitution System for Functional Analysis of Plant Solute Transporters
- The virescent-2 Mutation Inhibits Translation of Plastid Transcripts for the Plastid Genetic System at an Early Stage of Chloroplast Differentiation
- Efficient Transformation of Suspension-cultured Rice Cells Mediated by Agrobacterium tumefaciens
- Expression of Parsley Flavone Synthase I Establishes the Flavone Biosynthetic Pathway in Arabidopsis thaliana
- Construction of a Protein Library of Arabidopsis Transcription Factors Using a Wheat Cell-Free Protein Production System and Its Application for DNA Binding Analysis
- Novel Bacterial N-Acetyltransferase Gene for Herbicide Detoxification in Land Plants and Selection Maker in Plant Transformation
- In Vitro Protein Import of a Putative Amino Acid Transporter from Arabidopsis thaliana into Chloroplasts and Its Suborganellar Localization
- Alternative Processing of Arabidopsis Hsp70 Precursors during Protein Import into Chloroplasts
- In Vitro Fluorescent Analysis of Preprotein Import into Chloroplasts
- In Vitro Protein Import of a Putative Amino Acid Transporter from Arabidopsis thaliana into Chloroplasts and Its Suborganellar Localization
- Exploration of a Possible Partnership among Orphan Two-Component System Proteins in Cyanobacterium Synechococcus elongatus PCC 7942
- Characterization of the Plastidic Phosphate Translocators in the Inducible Crassulacean Acid Metabolism Plant Mesembryanthemum crystallinum