In silico identification and microarray analysis of human mucin-like genes
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概要
- 論文の詳細を見る
Mucins, which are highly O-glycosylated proteins, are involved in a variety of diseases. Most of the known mucins show little sequence conservation. To identify novel candidate mucins that might be helpful for diagnosis and therapy of diseases, bioinformatics approaches other than homology searches are required. We have developed two bioinformatics approaches that focus on either the amino acid compositions or the repeated sequences in regions carrying 0-linked glycans. Both approaches identify candidate mucins efficiently from the human reference sequence database. The former and latter approaches identified three and two novel mucin-like genes, respectively. We used Affymetrix GeneChip oligonucleotide microarrays to analyze the mucins identified from the database and found that three human mucin genes differed significantly in expression between normal tissues/cells and cancer cell lines. A combination of our bioinformatics approaches and the oligonucleotide microarray method will be useful for the efficient identification of candidate human mucins. Further study of the mucins in cancer tissues will facilitate the direct evaluation of their potential for cancer diagnosis and therapy.
- お茶の水女子大学の論文
著者
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Aburatani Hiroyuki
Genome Science Division Research Center For Advanced Science And Technology University Of Tokyo
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Sakamoto Yasunari
横浜市立大学 医学研究科消化器内科
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Yamanaka Shoji
横浜市立大学 医学部外科治療学
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Yamanaka Shoji
Department Of Surgery Yokohama City University School Of Medicine
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Yamanaka Shoji
横浜市立大学 医学研究科分子消化管内科学
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Yamamoto Shogo
東京大学先端科学技術研究センター ゲノムサイエンス分野
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Yamamoto Shogo
Genome Science Division Research Center For Advanced Science And Technology (rcast) University Of To
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Aburatani Hiroyuki
東京大学先端科学技術研究センター ゲノムサイエンス分野
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Aburatani Hiroyuki
Genome Science Division Center For Collaborative Research University Of Tokyo
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Nishi-Takaoka Chikako
Genaris, Inc.
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Nishi Tatsunari
Genaris, Inc.
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Shimamura Takahiro
Genome Science Division, Research Center for Advanced Science and Technology, The University of Toky
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Hippo Yoshitaka
Genome Science Division, Research Center for Advanced Science and Technology, The University of Toky
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Shimamura Takahiro
Genome Science Division Research Center For Advanced Science And Technology The University Of Tokyo
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Yamanaka Shoji
Division Of Anatomical And Surgical Pathology Yokohama City University Hospital
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Nishi Tatsunari
Genaris Inc.:genome Science Division Research Center For Advanced Science And Technology The Univers
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Nishi-takaoka Chikako
Genaris Inc.:genome Science Division Research Center For Advanced Science And Technology The Univers
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Hippo Yoshitaka
Genome Science Division Research Center For Advanced Science And Technology The University Of Tokyo
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