Gamma-Crosslinked Collagen Gel without Fibrils: Analysis of Structure and Heat Stability
スポンサーリンク
概要
- 論文の詳細を見る
This paper reports an analysis of the structure and heat stability of two different collagen gels: conventional collagen gel (neutral gel) and gel without collagen fibrils (acidic gel), previously reported. We performed differential scanning calorimetry (DSC), observations by scanning electron microscope (SEM), observations by atomic force microscope (AFM), and small angle X-ray scattering (SAXS). Collagen fibrils were clearly observed in the neutral gel but not in the acidic gel by both SEM and AFM. A clear endothermic peak was observed at 53–55 °C, representing disassembly of collagens in collagen fibrils in the neutral gel but not in the acidic gel. Only a small broad endothermic peak, at 35–43 °C, representing the deformation of the triple helical structure of collagen, was observed in the acidic gel. The SAXS pattern also suggested that the neutral gel had a more heterogeneous structure than the acidic gel. The experimental results described here are compatible with the model proposed in a previous paper, and indicate more clearly that the acidic gel has no collagen fibrils and has a different molecular assembly state of Type I collagen than the neutral gel.
- 社団法人 日本農芸化学会の論文
著者
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Hara Masayuki
Department of Biological Science, Graduate School of Science, Osaka Prefecture University
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Bessho Masahiko
Department of Biological Science, Graduate School of Science, Osaka Prefecture University
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SUZUKI Shiho
Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo
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KITAMURA Shinichi
Department of Biological Resource Chemistry, Kyoto Prefectural University
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KOSHIMIZU Naoki
Department of Biological Science, Graduate School of Science, Osaka Prefecture University
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YUGUCHI Yoshiaki
Faculty of Engineering, Osaka Electro-Communication University
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