Some Factors Affecting Cellulose Degradation with Pellicularia filamentosa Cellulases : Studies on the Re-utilization of Cellulosic Resources (II)

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Cellulose degradation with a crude cellulase preparation obtained from the culture filtrate of Pellicularia filamentosa strain FERM-P 1797 rapidly reached a plateau at a low level of degradation. To explain and prevent this phenomenon, several factors affecting cellulase activities were incestigated for three cellulase components, I-A-3,I-A-4,and I-A-5,fractionated from the crude cellulase. Marked lowering of the cellulose degradation rate was observed in every component tested, as in the crude cellulase. However, no evidence was obtained that denaturation or product inhibition are involved under the conditions tested. In the cas of I-A-4,a component of high substrate specificity toward crystalline cellulose, the sole condition for the phenomenon is the coexistence of cellulase and cellulose. The addition of at least 100 μg of non-cellulase protein per ml of reaction mixture prevented the lowering of activity. In the case of I-A-5,a component of high substrate specificity toward CM-cellulose, the occurrence of a gas-liquid interface under the coexistence of cellulase and substrate may be one cause of the lowering of activity. The phenomenon could be prevented by addition of non-cellulase protein with CM-cellulose as substrate, but not with crystalline cellulose. I-A-3,a component of high substrate specificity toward crystalline cellulose and CM-cellulose, showed the combined properties of the other two components with respect to the causes and methods of prevention of the lowering of activity.
公益社団法人日本生物工学会の論文
1978-04-25

Some Factors Affecting Cellulose Degradation with Pellicularia filamentosa Cellulases : Studies on the Re-utilization of Cellulosic Resources (II)

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