Preparation of a Novel Thermo-Responsive Polymer and Its Use as a Carrier for Immobilization of Thermolysin
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概要
- 論文の詳細を見る
A copolymer (GNM) composed of three monomers, glycidyl methacrylase (GMA), N-isopropyl acrylamide(NIPAm), and methacrylamide (MAm), was prepared as a new thermo-responsive polymer for immobilization of an enzyme which has a maximal activity at high temperature. The polymer (GNM-75) prepared at the mole percentage ratio of GMA : NIPAm : MAm = 15 : 75.5 : 7.5 has a critical temperature (CT) of around 42℃, at which its solubility exhibits a sharp change. The CT of GNM-75 is 10℃ higher than those of poly(NIPAm) and poly(GMA/NIPAm) reported previously [Hoshino K. et al. : J. Chem. Eng. Jpn., 20,569-574,1992]. Thermolysin was covalently coupled with GNM-75 at pH 10.5. The thermolysin immobilized on GNM-75 showed a good solubility response : the immobilized thermolysin in the buffer solution (pH 8.0) became soluble upon decrease of the temperature of the buffer solution to below 44℃, was completely insoluble above 54℃ and could be recovered efficiently by centrifugation. Moreover, the CT of the immobilized thermolysin was decreased by about 4.6℃ for each 20 g/l increase in the ammonium sulfate concentration of the mixture. The specific activity of the immobilized thermolysin at 40℃ was higher than that of thermolysin immobilized on a conventional thermo-responsive polymer (GMA-NIPAm). For its repeated use in hydrolysis of α-casein, the immobilized thermolysin was recovered from the reaction mixture, by two methods. When the reaction was finished, the immobilized thermolysin was insolubilized either by elevation of the temperature of the mixture from 40℃ to 55℃, or by adjustment of the ammonium sulfate concentration of the mixture from 0 g/l to 60 g/l at 40℃ to 55℃, or by adjustment of the ammonium sulfate concentration of the mixture from 0 g/l to 60 g/l at 40℃. The thermo-responsive polymer possessing a relatively high CT was found to be useful as a carrier for immobilization of an enzyme which has a maximal activity at high temperature such as thermolysin.
- 社団法人日本生物工学会の論文
- 1997-03-25
著者
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Taniguchi Masayuki
Department of Materials Science and Technology, Niigata University
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Hoshino Kazuhiro
Department of Materials Systems Engineering and Life Science, Toyama University
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Taniguchi M
Fac. Engineering Niigata Jpn
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Taniguchi M
Department Of Material Science And Technology Faculty Of Engineering
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Taniguchi M
Department Of Materials Science And Technology Niigata University
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Taniguchi Masayuki
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Taiyouji Mayumi
Gaduate School Of Science And Technology Niigata University:food Research Center Niigata Agricultura
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Taniguchi Masayuki
Department Of Materials Science And Technology Niigata University
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Taniguchi Masayuki
Gaduate School Of Science And Technology Niigata University:food Research Center Niigata Agricultura
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Hoshino Kazuhiro
Department Of Materials Systems Engineering And Life Science Toyama University
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Tanaka Mitsuo
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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MOROHASHI Shoichi
Department of Chemical Engineering, Faculty of Engineering, Toyama University
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KAWABERI HIROYUKI
Department of Chemical and Biochemical Engineering, Faculty of Engineering, Toyama University
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TAKEDA YOSHIYASU
Department of Chemical and Biochemical Engineering, Faculty of Engineering, Toyama University
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SAKAKURA TOSHISUKE
Department of Chemical and Biochemical Engineering, Faculty of Engineering, Toyama University
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Takeda Yoshiyasu
Department Of Chemical And Biochemical Engineering Faculty Of Engineering Toyama University
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Kawaberi Hiroyuki
Department Of Chemical And Biochemical Engineering Faculty Of Engineering Toyama University
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Sasakura T
Toyama Univ. Toyama
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Taniguchi Masayuki
Department Of Cardiology Jikei University School Of Medicine Daisan Hospital
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Hoshino Kazuhiro
Section Of Functional Molecule Production Systems Field Of Nano And Functional Material Sciences Gra
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Morohashi S
Depertment Of System Engineering Of Materials And Life Science Toyama University
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Morohashi Shoichi
Section Of Functional Molecule Production Systems Field Of Nano And Functional Material Sciences Gra
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Morohashi Shoichi
Department Of Chemical Engineering Faculty Of Engineering Toyama University
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Morohashi Shoichi
Department of Chemical and Biochemical Engineering, Toyama University
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Hoshino Kazuhiro
Department of Chemical and Biochemical Engineering, Toyama University
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Takahashi Masafumi
Department of Materials Science and Technology, Niigata University
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