Repeated Utilization of β-Glucosidase Immobilized on a Reversibly Soluble-Insoluble Polymer for Hydrolysis of Phloridzin as a Model Reaction Producing a Water-Insoluble Product

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概要

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• β-Glucosidase immobilized on a reversibly soluble-insoluble polymer, G-AS, was utilized for the hydrolysis of phloridzin. The solubility of the immobilized enzyme was dependent on pH and temperature of the solution : its responsive ranges was between pH 4.0 and 5.0 at 50°C and between pH 3.5 and 4.5 at 4°C. Soluble G-AS had a higher specific activity toward phloridzin than that of the conventional enzyme immobilized on a water-insoluble carrier. For the hydrolysis of phloridzin at a concentration 5 g/l (the reaction produces a water-insoluble product from a water-soluble substrate), G-AS was used repeatedly and was easily separated by centrifugation from the reaction mixture containing a water-insoluble product. In addition, for the hydrolysis of phloridzin at a concentration of 50 g/l (the reaction produces a water-insoluble product from a slightly water-soluble substrate), G-AS was also used repeatedly and was separated for reuse from the mixture of unreacted phloridzin, glucose, and phloretin. The reaction method using such a reversibly soluble-insoluble enzyme in a heterogeneous reaction with a water-insoluble product is a potential procedure for repeatedly utilizing the enzyme and separating the water-insoluble product from the suspended mixture.

• 社団法人日本生物工学会の論文
• 1996-09-25

著者

• Taniguchi Masayuki

  Department of Materials Science and Technology, Niigata University

• Hoshino Kazuhiro

  Department of Materials Systems Engineering and Life Science, Toyama University

• Taniguchi M

  Fac. Engineering Niigata Jpn

• Taniguchi M

  Department Of Material Science And Technology Faculty Of Engineering

• Taniguchi M

  Department Of Materials Science And Technology Niigata University

• Taiyouji Mayumi

  Gaduate School Of Science And Technology Niigata University:food Research Center Niigata Agricultura

• Taniguchi Masayuki

  Department Of Materials Science And Technology Niigata University

• Hoshino Kazuhiro

  Department Of Materials Systems Engineering And Life Science Toyama University

• Tanaka Mitsuo

  Department Of Food Science And Technology Faculty Of Agriculture Kyoto University

• MOROHASHI Shoichi

  Department of Chemical Engineering, Faculty of Engineering, Toyama University

• SASAKURA Toshisuke

  Department of Chemical and Biochemical Engineering, Toyama University

• UEOKA HITOMI

  Department of Chemical and Biochemical Engineering, Faculty of Engineering, Toyama University

• OHKUWA MAYUMI

  Department of Chemical and Biochemical Engineering, Faculty of Engineering, Toyama University

• CHIDA CHIE

  Department of Chemical and Biochemical Engineering, Faculty of Engineering, Toyama University

• Sasakura T

  Toyama Univ. Toyama

• Ueoka Hitomi

  Department Of Chemical And Biochemical Engineering Faculty Of Engineering Toyama University

• Taniguchi Masayuki

  Department Of Cardiology Jikei University School Of Medicine Daisan Hospital

• Hoshino Kazuhiro

  Section Of Functional Molecule Production Systems Field Of Nano And Functional Material Sciences Gra

• Morohashi S

  Depertment Of System Engineering Of Materials And Life Science Toyama University

• Morohashi Shoichi

  Section Of Functional Molecule Production Systems Field Of Nano And Functional Material Sciences Gra

• Morohashi Shoichi

  Department Of Chemical Engineering Faculty Of Engineering Toyama University

• Chida Chie

  Department Of Chemical And Biochemical Engineering Faculty Of Engineering Toyama University

• Ohkuwa Mayumi

  Department Of Chemical And Biochemical Engineering Faculty Of Engineering Toyama University

• Morohashi Shoichi

  Department of Chemical and Biochemical Engineering, Toyama University

• Hoshino Kazuhiro

  Department of Chemical and Biochemical Engineering, Toyama University

• SASAKURA TOSHISUKE

  Department of Chemical and Biochemical Engineering, Faculty of Engineering, Toyama University

• Takahashi Masafumi

  Department of Materials Science and Technology, Niigata University

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