Repeated Batch Conversion of Raw Starch to Ethanol Using Amylase Immobilized on a Reversible Soluble-autoprecipitating Carrier and Flocculating Yeast Cells(Microbiology & Fermentation Industry)
スポンサーリンク
概要
- 論文の詳細を見る
A raw-starch-digesting amylase, Dabiase K-27, was immobilized covalently on an enteric coating polymer (hydroxypropyl methylcellulose acetate succinate : AS) as a carrier which is autoprecipitating in an insoluble state below pH 4 as well as reversibly soluble-insoluble depending on pH. Dabiase immobilized on AS (D-AS) showed a sharp response of solubility to slight changes of pH without decrease in enzymatic activity. Moreover, D-AS in an insoluble state had good properties of sedimentation and a large portion of D-AS spontaneously precipitated after 10min at pH 4. D-AS was used successively for repeated ethanol production from raw starch, in which D-AS and flocculating yeast cells were separated simultaneously from a product solution by sedimentation in a reactor with a conical bottom. In the five batches of 10% raw starch, the total amount of ethanol produced from 150g of raw starch was 61g, a value of which corresponds to the average ethanol productivity of 0.85g/l/hr. The repeated ethanol production by a combination of D-AS and flocculating yeast cells is a promising procedure for effectively using the enzyme and recovering the product solution economically in a heterogeneous culture system containing a solid substrate.
- 社団法人日本農芸化学会の論文
- 1989-07-23
著者
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Taniguchi Masayuki
Department of Materials Science and Technology, Niigata University
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HOSHINO Kazuhiro
Graduate School of Natural Science and Technology, Niigata University
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FUJII Michihiro
Department of Chemical Engineering, Faculty of Engineering, Niigata University
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Taniguchi M
Fac. Engineering Niigata Jpn
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Taniguchi M
Department Of Material Science And Technology Faculty Of Engineering
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MARUMOTO Hideji
Department of Chemical Engineering, Faculty of Engineering, Niigata University
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Taiyouji Mayumi
Gaduate School Of Science And Technology Niigata University:food Research Center Niigata Agricultura
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Marumoto Hideji
Department Of Chemical Engineering Faculty Of Engineering Niigata University
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Taniguchi Masayuki
Department Of Materials Science And Technology Niigata University
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Hoshino Kazuhiro
Department Of Materials Systems Engineering And Life Science Toyama University
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Tanaka Mitsuo
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Fujii Michihiro
Department Of Chemistry And Chemical Engineering Niigata University
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Fujii Michihiro
Department Of Chemical Engineering Faclty Of Engineering Niigata University
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Taniguchi Masayuki
Department Of Cardiology Jikei University School Of Medicine Daisan Hospital
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Takahashi Masafumi
Department of Materials Science and Technology, Niigata University
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