Hydrolysis of Starchy Materials by Repeated Use of an Amylase Immobilized on a Novel Thermo-Responsive Polymer
スポンサーリンク
概要
- 論文の詳細を見る
A copolymer of methacrylic acid (MAA) and N-isopropyl acrylamide (NIPAM) was used as a novel, reversibly soluble-insoluble support whose solubility changes depending on the temperature of the solution. Amylase (Dabiase K-27) immobilized covalently on the thermo-responsive polymer showed good solubility response : the immobilized enzyme (D-MN) was in a soluble state below 32℃, but insoluble above 42℃. D-MN in a soluble state has a high specific activity for the hydrolysis of soluble or uncooked starch. The solubility response of D-NM to changes in the temperature of the solution was more sensitive when 0.5% NaCl was added to a buffer solution (pH4.5) with D-MN than in the buffer solution without NaCl. D-MN was used successively for repeated hydrolysis reactions of soluble and uncooked starches, in which D-MN was insolubilized either by changing the temperature of the reaction mixture from 30℃ to 36℃ with 0.5% NaCl or by adjusting the NaCl concentration of the reaction mixture from 0% to 1% at 30℃. In the repeated hydrolysis, glucose was produced successively from the soluble and uncooked starches, and D-MN could be repeatedly used after being recovered from the reaction product by centrifugation at the end of each batchwise hydrolysis.
- 社団法人日本生物工学会の論文
- 1994-04-25
著者
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Taniguchi Masayuki
Department of Materials Science and Technology, Niigata University
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Hoshino Kazuhiro
Department of Materials Systems Engineering and Life Science, Toyama University
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FUJII Michihiro
Department of Chemical Engineering, Faculty of Engineering, Niigata University
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Taniguchi M
Fac. Engineering Niigata Jpn
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Taniguchi M
Department Of Material Science And Technology Faculty Of Engineering
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Taiyouji Mayumi
Gaduate School Of Science And Technology Niigata University:food Research Center Niigata Agricultura
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Taniguchi Masayuki
Department Of Materials Science And Technology Niigata University
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Hoshino Kazuhiro
Department Of Materials Systems Engineering And Life Science Toyama University
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Tanaka Mitsuo
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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SASAKURA Toshisuke
Department of Chemical and Biochemical Engineering, Toyama University
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KATAGIRI MASAFUMI
Department of Chemistry and Chemical Engineering, Niigata University
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Sasakura T
Toyama Univ. Toyama
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Fujii Michihiro
Department Of Chemistry And Chemical Engineering Niigata University
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Fujii Michihiro
Department Of Chemical Engineering Faclty Of Engineering Niigata University
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Taniguchi Masayuki
Department Of Cardiology Jikei University School Of Medicine Daisan Hospital
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Hoshino Kazuhiro
Section Of Functional Molecule Production Systems Field Of Nano And Functional Material Sciences Gra
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Katagiri Masafumi
Department Of Chemistry And Chemical Engineering Niigata University
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Hoshino Kazuhiro
Department of Chemical and Biochemical Engineering, Toyama University
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SASAKURA TOSHISUKE
Department of Chemical and Biochemical Engineering, Faculty of Engineering, Toyama University
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Takahashi Masafumi
Department of Materials Science and Technology, Niigata University
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