Continuous Simultaneous Saccharification and Fermentation of Delignified Rice Straw by a Combination of Two Reversibly Soluble-Autoprecipitating Enzymes and Pentose-Fermenting Yeast Cells.

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概要

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• Direct ethanol production from delignified rice straw was performed continuously using a combination of two reversibly soluble-autoprecipitating enzymes, [Meicelase and Cellulase Y-NG were individually immobilized on hydroxypropyl methylcellulose acetate succinate (AS-L)] and <I>Pichia stipitis</I> cells. Cellulase Y-NG immobilized on AS-L (X-AS) as well as Meicelase immobilized on AS-L (M-AS) proposed previously (Hoshino <I>et al</I>., 1994) exhibited a sharper response for the solubility depending on the pH changes; insoluble below 3.5 and soluble above 5.0. When delignified rice straw was hydrolysed using two immobilized enzymes at a ratio of X-AS/M-AS = l, the amount of soluble sugars obtained was higher than that using M-AS alone. Moreover, both immobilized enzymes in an insoluble state had good self-sedimentation properties and were coprecipitated with a large portion of <I>P. stipitis</I> cells.<BR>Ethanol production was performed continuously by a reactor system equipped with a mixing vessel and a separation vessel. Both immobilized enzymes and the yeast cells were separated continuously from a solution containing ethanol by co-precipitation in the separation vessel and they was returned to a main reactor for reuse. In continuous ethanol production from the rice straw, high ethanol productivity of up to 0.113 kg/(m<SUP>3</SUP>·h) was achieved at a dilution rate of 0.050 h<SUP>-1</SUP>. This continuous fermentation system suggests potential for effective ethanol production from delignified rice straw.

• The Society of Chemical Engineers, Japanの論文

著者

• SASAKURA Toshisuke

  Department of Chemical and Biochemical Engineering, Toyama University

• Chida Chie

  Department Of Chemical And Biochemical Engineering Faculty Of Engineering Toyama University

• Morohashi Shoichi

  Department of Chemical and Biochemical Engineering, Toyama University

• Hoshino Kazuhiro

  Department of Chemical and Biochemical Engineering, Toyama University

• SASAKURA TOSHISUKE

  Department of Chemical and Biochemical Engineering, Faculty of Engineering, Toyama University

• Taniguchi Masayuki

  Depeartment of Material Science and Techno1gy, Niigata University

• Fujii Michihiro

  Niigata College of Techno1gy University

• Yamasaki Hiroshi

  Department of Chemical and Biochemical Engineering, Toyama University

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