Isolation and Characterization of cDNAs That Encode Eleven Small GTP-Binding Proteins from Pisum sativum
スポンサーリンク
概要
- 論文の詳細を見る
Eleven cDNA clones (pra1 to pra9A, pra9B, and pra9C) were isolated from a pea (Pisum sativum) leaf cDNA library which were similar to small GTP-binding proteins. These cDNAs en-coded proteins of 22-25 kDa, which exhibited 45-92% identity to one another at the amino acid level. The putative proteins included the characteristic sequences of ras-related small GTP-binding proteins: four conserved domains involved in binding of GTP/GDP; an effector domain; and cysteine residues at the COOH-terminus. Indeed, the pra6 protein, expressed in Escherichia coli, clearly showed GTP-binding activity. Phylogenetic analysis showed that these clones can be classified into two subgroups: proteins encoded by pra1 to pra7 being related to ypt3 and rab11 proteins; and proteins encoded by pra8. pra9A, pra9B, and pra9C being related to YPT1 and rab1 proteins. The effector sequences in these two subgroups were different. RNA gel blot analysis showed that most of the corresponding genes are differentially expressed in pea leaves and roots. Variations in expression were also observed for structurally related genes.
- 日本植物生理学会の論文
著者
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Sasaki Y
Kyoto Univ. Kyoto Jpn
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Sasaki Yukiko
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Sasaki Yukiko
Department Of Anesthesiology Kitano Hospital
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Murai Nobuhiko
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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MATSUNO RYUICHI
Department of Food Science and Technology, Faculty of Agriculture, Kyoto University
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佐々木 幸子
京都大学農学部
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Nagano Yukio
Department Of Food Science And Technology Faculty Of Agriculture Kyoto University
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Matsuno Ryuichi
Deparment Of Food Science And Technology Faculty Of Agriculture Kyoto University
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SASAKI Yukiko
Department of Anesthesia, The Tazuke Kofukai Medical Research Institute Kitano Hospital
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