Refolding of Denatured/Reduced Lysozyme at High Concentration with Diafiltration

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概要

• 論文の詳細を見る

• Refolding of reduced and denatured protein in vitro has been an important issue for both basic research and applied biotechnology. Refolding at low protein concentration requires large volumes of refolding buffer. Among various refolding methods, diafiltration is very useful to control the denaturant and red/ox reagents in a refolding solution. We constructed a refolding procedure of high lysozyme concentration (0.5-10mg/ml) based on the linear reduction of the urea concentration during diafiltration under oxygen pressure. When the urea concentration in the refolding vessel was decreased from 4M with a rate of 0.167M/h, the refolding yields were 85% and 63% at protein concentrations, 5mg/ml and 10mg/ml, respectively, after 11h. This method gave a high productivity of 40.1μm/h of the refolding lysozyme. The change in refolding yields during the diafiltration could be simulated using the model of Hevehan and Clark.

• 社団法人日本農芸化学会の論文
• 2000-06-23

著者

• 吉井 英文

  鳥取大学大学院工学研究科 化学・生物応用工学専攻

• Linko P

  Department Of Chemical Technology Helsinki University Of Technology

• Linko Yu-yen

  Department Of Chemical Technology Helsinki University Of Technology

• ITO Daisuke

  Department of Surgery and Surgical Basic Science, Kyoto University

• Furuta Takeshi

  Tottori University

• YOSHII Hidefumi

  Department of Biotechnology, Tottori University

• FURUTA Takeshi

  Department of Biotechnology, Tottori University

• LINKO Pekka

  Department of Chemical Technology, Helsinki University of Technology

• YONEHARA Takahiro

  Department of Biotechnology, Tottori University

• YONEHARA Takehiro

  Department of Biotechnology, Tottori University

• Yoshii H

  Tottori University

• Yoshii H

  Tottori Univ. Tottori Jpn

• Yoshii H

  Toyama National Coll. Technol. Toyama Jpn

• Yoshii Hidefumi

  Department Of Biochemical Engineering Toyama National College Of Technology

• Furuta T

  Tottori University

• Furuta Takeshi

  Department Of Biotechnology Faculty Of Engineering Tottori University

• Ito D

  Graduate School Of Engineering Osaka Prefecture University Department Of Applied Materials Science

• Linko Pekka

  Department Of Chemical Technology Helsinki University Of Technology

• Yoshii Hidefumi

  Department Of Applied Biological Science Kagawa University

• Yonehara Takehiro

  Department Of Biotechnology Tottori University

• Ito Daisuke

  Department Of Mechanical Science And Engineering Nagoya University

• Ito Daisuke

  Department Of Applied Materials Science. Graduate School Of Eneineerine. Osaka Prefecture University

• Ito Daisuke

  Department Of Advanced Bioscience Faculty Of Agriculture Kinki University

• ITO Daisuke

  Department of Biotechnology, Tottori University

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