Enzyme Immunoassay of Thyrotropin Releasing Hormone (TRH)
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概要
- 論文の詳細を見る
A sensitive and specific double-antibody enzyme immunoassay (EIA) for a thyrotropin releasing hormone (TRH)-like immunoreactive substance has been developed. In order to synthesize TRH-labeled β-D-galactosidase (β-gal), a newly devised TRH derivative, pGlu-His-Pro-NH-(CH_2)_6-NH_2 (TRH-Hex), was employed. TRH-Hex was linked to β-gal by the N-(ε-maleimidiocaproyloxy) succinimide coupling procedure. For competitive reactions, the TRH antibody was incubated with standard TRH and TRH-Hex-β-gal (delayed addition). Free and antibody-bound enzyme hapten were separated by using an anti-rabbit immunoglobulin G coated immunoplate. Activity of the enzyme on the plate was fluorometrically determined. The present immunoassay allows detection of 0.8 to 100 pmol/well of TRH.
- 公益社団法人日本薬学会の論文
- 1992-08-25
著者
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大高 章
Faculty of Pharmaceutical Sciences, Kyoto University
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藤井 信孝
Faculty of Pharmaceutical Sciences, Kyoto University
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武山 正治
Department of Clinical Pharmacy, Oita Medical University
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大高 章
徳島大学大学院ヘルスバイオサイエンス研究部
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大高 章
京都大学 薬
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藤井 信孝
京都大学大学院薬学研究科 医薬創成情報科学専攻
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武山 正治
Department Of Clinical Pharmacy Oita Medical University
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大高 章
京大 大学院薬学研究科
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