Enzyme Immunoassay of a Substance P-like Immunoreactive Substance in Human Plasma and Saliva
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概要
- 論文の詳細を見る
A sensitive and specific double-antibody enzyme immunoassay (EIA) for a substance P (SP)-like immunoreactive substance (SP-IS) was developed. For competitive reactions, the SP-antibody was incubated with SP standard (or sample) and β-D-galactosidase labeled Tyr^8-SP (delayed addition). Free and antibody-bound enzyme hapten were separated by using an anti-rabbit immunoglobulin G coated immunoplate. Activity of the enzyme on the plate was fluorometrically determined. The present immunoassay allows detection of 0.4 to 10fmol/well of SP. Using the present EIA, SP-ISs in human saliva and plasma were determined. The level of SP-IS in human saliva was about 7pmol/l, which was almost three times higher than that in human plasma.
- 公益社団法人日本薬学会の論文
- 1990-12-25
著者
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藤井 信孝
京都大学薬学部
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森 一生
大分大学医学部附属病院薬剤部
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北川 幸己
徳島大学薬学部
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藤井 信孝
Faculty of Pharmaceutical Sciences, Kyoto University
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北川 幸己
Faculty of Pharmaceutical Sciences, Kyoto University
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武山 正治
Department of Clinical Pharmacy, Oita Medical University
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北川 幸己
Faculty Of Pharmaceutical Sciences University Of Tokushima
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森 一生
Department of Clinical Pharmacy, Oita Medical University
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高山 房子
大分医大薬剤部
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武山 正治
Department Of Clinical Pharmacy Oita Medical University
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藤井 信孝
京大 薬
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高山 房子
Department of Hospital Pharmacy, Medical College of Oita
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近藤 和宏
Department of Hospital Pharmacy, Medical College of Oita
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高山 房子
大分医科大学薬効薬物動態治療応用講座
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近藤 和宏
Department Of Hospital Pharmacy Medical College Of Oita
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藤井 信孝
京都大学
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Mori Kazuo
Department Of Clinical Pharmacy Oita Medical University
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