Micro-Enzyme Immunoassay of Vasoactive Untestinal Polypeptide (VIP)-like Immunoreactive Substance in Bovine Milk
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概要
- 論文の詳細を見る
A sensitive and specific enzyme immunoassay for vasoactive intestinal polypeptide (VIP)-like immunoreactivity was developed with the use of synthetic carboxy-terminal(C-terminal) fragment (residue 11-28) of porcine VIP conjugated with β-D-galactosidase and a second antibody-coated immunoplate. Using 4-methylumbelliferyl β-D-galactopyranoside as a fluorogenic substrate, the minimum amount of VIP-like immunoreactive substance (VIP-IS) detectable by this method was 0.1 fmol/well (2.5 pmol/l). The level of VIP-IS in bovine foremilk was above 100 pmol/l, which was more then eightfold higher than that in normal milk.
- 公益社団法人日本薬学会の論文
- 1990-04-25
著者
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藤井 信孝
京都大学薬学部
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藤井 信孝
Faculty of Pharmaceutical Sciences, Kyoto University
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矢島 治明
Faculty of Pharmaceutical Sciences, Kyoto University
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武山 正治
Department of Clinical Pharmacy, Oita Medical University
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高山 房子
大分医大薬剤部
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武山 正治
Department Of Clinical Pharmacy Oita Medical University
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矢島 治明
京都大学
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矢島 治明
京大・薬
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藤井 信孝
京大 薬
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矢島 治明
Faculty Of Pharmaceutical Sciences Kyoto University
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高山 房子
Department of Hospital Pharmacy, Medical College of Oita
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近藤 和宏
Department of Hospital Pharmacy, Medical College of Oita
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若山 香都美
Department of Hosiptal Pharmacy, Medical College of Oita
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高山 房子
大分医科大学薬効薬物動態治療応用講座
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近藤 和宏
Department Of Hospital Pharmacy Medical College Of Oita
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若山 香都美
大分医科大学附属病院薬剤部
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藤井 信孝
京都大学
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