Studies on Peptides. CXXV. 3-(3-p-Methoxybenzylthiopropionyl)-thiazolidine-2-thione and Its Analogs as Reagents for the Introduction of the Mercapto Group into Peptides and Proteins
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概要
- 論文の詳細を見る
A new SH-introducing reagent, 3-(3-p-methoxybenzylthiopropionyl) thiazolidine-2-thione (MTPTT), was prepared by condensation of 3-p-methoxybenzylthiopropionic acid and thiazolidine-2-thione using DCC. Reaction of MTPTT with peptides can be monitored by following the disappearance of the pale yellow color of the reagent. As an example, synthetic human growth hormone-releasing factor (hGRF-40) was treated with MTPTT, followed by 1M trifluoromethanesulfonic acid-thioanisole/trifluoroacetic acid. The resulting HS-CH_2CH_2-CO-(hGRF-40) was conjugated with bovine serum albumin (BSA) by using a newly introduced heterobifunctional conjugating reagent, 2,4-dinitrophenyl p-(β-nitrovinyl) benzoate for antigen preparation. Four other similar SH-introducing reagents were also prepared.
- 公益社団法人日本薬学会の論文
- 1985-01-25
著者
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藤井 信孝
Faculty of Pharmaceutical Sciences, Kyoto University
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赤路 健一
Faculty of Pharmaceutical Sciences
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矢島 治明
京都大学
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矢島 治明
京大・薬
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藤井 信孝
京大 薬
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矢島 治明
Faculty Of Pharmaceutical Sciences Kyoto University
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赤路 健一
Faculty Of Pharmaceutical Sciences Kyoto University
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林 良雄
Faculty Of Pharmaceutical Sciences Kyoto University
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