Immuno-affinity Purification of Specific Antibodies against Vasoactive Intestinal Polypeptide (VIP) on VIP (1-10)-Linked Polydimethylacrylamide Resin
スポンサーリンク
概要
- 論文の詳細を見る
A novel method for immuno-affinity purification of specific antibodies against amino-terminal (N-terminal) porcine vasoactive inestinal polypeptide (p-VIP) was developed. The antiserum VP (No. 6203) elicited by p-VIP-immunoglobulin G (IgG) conjugate was heterogenous and reacted not only with the N-terminal fragments of p-VIP but also with carboxy-terminal (C-terminal)fragments. To obtain specific antibodies against the N-terminal fragment of p-VIP, antiserum VP was purified by column chromatography on N-terminal decapeptide (p-VIP 1-10)-linked polydimethylacrylamide resin. The antibody thus obtained was highly specific to the N-terminal sequence of p-VIP and hardly reacted with the C-terminal fragments of VIP in enzyme-liked immunosorbent assay.
- 公益社団法人日本薬学会の論文
- 1989-03-25
著者
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藤井 信孝
京都大学薬学部
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大高 章
Faculty of Pharmaceutical Sciences, Kyoto University
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藤井 信孝
Faculty of Pharmaceutical Sciences, Kyoto University
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矢島 治明
Faculty of Pharmaceutical Sciences, Kyoto University
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武山 正治
Department of Clinical Pharmacy, Oita Medical University
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大高 章
徳島大学大学院ヘルスバイオサイエンス研究部
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大高 章
京都大学 薬
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武山 正治
Department Of Clinical Pharmacy Oita Medical University
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大高 章
京大 大学院薬学研究科
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矢島 治明
京都大学
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矢島 治明
京大・薬
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藤井 信孝
京大 薬
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矢島 治明
Faculty Of Pharmaceutical Sciences Kyoto University
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波多野 美香
Faculty of Pharmaceutical Sciences, Kyoto University
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波多野 美香
Faculty Of Pharmaceutical Sciences Kyoto University
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藤井 信孝
京都大学
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