Studies on Peptides. CIV. Synthesis of Ribonuclease S'

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概要

• 論文の詳細を見る

• Ribonuclease (RNase) S' was prepared by the combination of synthetic S-peptide and synthetic S-protein. The S-peptide was synthesized by the azide condensation of three fragments, (1-8), (9-13) and (14-20), followed by deprotection with 1 M trifluoromethanesulfonic acid-thioanisole in trifluoroacetic acid. The same reagent was employed to remove all protecting groups from the protected S-protein. In order to establish the disulfide bonds of the deprotected S-protein, glutathione-mediated air oxidation was performed in the presence of the S-peptide. The product was purified by affinity chromatography followed by ion-exchange chromatography on sulfopropyl-Sephadex to afford RNase S' in 0.5% yield from the protected S-protein.

• 公益社団法人日本薬学会の論文
• 1981-07-25

著者

• 藤井 信孝

  Faculty of Pharmaceutical Sciences, Kyoto University

• 矢島 治明

  京都大学

• 矢島 治明

  Faculty Of Pharmaceutical Sciences Kyoto University

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