Enzyme Immunoassay of Elcatonin in Human Plasma
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概要
- 論文の詳細を見る
A sensitive and specific double-antibody enzyme immunoassay (EIA) for detecting an elcatonin-like immunoreactive substance (ECT-IS) in human plasma has been developed. In competitive reactions, the ECT antibody was incubated with a plasma sample (or ECT standard) and β-D-galactosidase-linked synthetic ECT. Free and antibody-bound enzymes were separated using an anti-rabbit IgG-coated immunoplate. Enzyme activity on the plate was determined by fluorescence analysis. This immunoassay allows the detection of 20 to 300 fmol/ml (67 to 1000 pg/ml) of ECT. The EIA was applied to determine the pharmacokinetic behavior of ECT after a single intramuscular administration (20 IU). The maximum level was achieved 30 min after administration, at approximately 30 pg ECT/ml of plasma.
- 社団法人日本薬学会の論文
- 1995-06-15
著者
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猪川 和朗
大分医科大学医学部附属病院薬剤部
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森 一生
大分大学医学部附属病院薬剤部
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武山 正治
Department of Clinical Pharmacy, Oita Medical University
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猪川 和朗
Department of Clinical Pharmacy, Oita Medical University
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衛藤 いづみ
Department of Clinical Pharmacy, Oita Medical University
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森 一生
Department of Clinical Pharmacy, Oita Medical University
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衛藤 いづみ
Department Of Clinical Pharmacy Oita Medical University
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武山 正治
Department Of Clinical Pharmacy Oita Medical University
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Mori Kazuo
Department Of Clinical Pharmacy Oita Medical University
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