Enzyme Linked Immunosorbent Assay for β-Endorphin in Human Plasma

概要

論文の詳細を見る
We established a highly sensitive and specific double-antibody enzyme linked immunosorbent assay for β-endorphin (β-EP). For competitive reactions, the β-EP-antibody was incubated with β-EP standard (or sample) and β-D-galactosidase-labeled β-EP (delayed addition). Free and antibody-bound labeled antigen were separated by using an anti-rabbit immunoglobulin G coated immunoplate. The enzyme activity on the plate was fluorometrically determined. The minimal detection limit was approximately 0.4 fmol/well (10 pmol/l). Using this assay system, β-EP-like immunoreactivity (-LI) in human plasma was determined. The level of β-EP-LI in extracted human plasma from 6 normal subjects was 2.44±0.68 pmol/l. High performance liquid chromatography analysis of the plasma of a normal subject revealed a single immunoreactive form which eluted with the same retention time as that of synthetic β-EP.
社団法人日本薬学会の論文
1992-07-25