Enzyme Immunoassay of Motilin in Human Plasma
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概要
- 論文の詳細を見る
A sensitive and specific double-antibody enzyme-linked immunoassay (EIA) for detecting a motilin-like immunoreactive substance (M-IS) in human plasma has been developed. In competitive reactions, the motilin antibody was incubated with a plasma sample (or motilin standard) and β-D-galactosidase-linked synthetic motilin. Free and antibody-bound enzymes were separated using an anti-rabbit IgG-coated immunoplate. Enzyme activity on the plate was determined by fluorophotometric analysis. This immunoassay allows the detection of 20 to 200 fmol/ml (54 to 540 pg/ml) of motilin. The mean level of M-IS detected in human plasma was 53.9±25.6 pg/ml.
- 公益社団法人日本薬学会の論文
- 1994-03-15
著者
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森 一生
大分大学医学部附属病院薬剤部
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河野 亜由美
Department of Hospital Pharmacy, Medical College of Oita
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朝倉 俊治
大分赤十字病院薬剤部
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森 一生
Department of Clinical Pharmacy, Oita Medical University
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武山 正治
Department Of Clinical Pharmacy Oita Medical University
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河野 亜由美
大分医科大学医学部附属病院薬剤部
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朝倉 俊治
Department of Clinical Pharmacy, Oita Medical University
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Mori Kazuo
Department Of Clinical Pharmacy Oita Medical University
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