Enzyme Immunoassary of Gastrin in Human Plasma
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概要
- 論文の詳細を見る
A sensitive and specific double-antibody enzyme immunoassay (EIA) for a gastrin-like immunoreactive substance (G-IS) in human plasma was developed. For competitive reactions, the gastrin antibody was incubated with gastrin standard (or sample) and β-D-galactosidase labeled synthetic C-terminal gastrin I fragment (residue 2-17). Free and antibody-bound enzyme hapten were separated using an anti-rabbit IgG coated immunoplate. Activity of the enzyme on the plate was fluorometrically determined. The present immunoassay allows detection of 1 to 20 fmol/ml (2.1 to 42 pg/ml) of gastrin. The levels of G-IS determined in human plasma were 7.8±1.6 pg/ml before lunch and 26.4±8.4 pg/ml after lunch.
- 社団法人日本薬学会の論文
- 1993-12-15
著者
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森 一生
大分大学医学部附属病院薬剤部
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武山 正治
Department of Clinical Pharmacy, Oita Medical University
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松尾 博文
Department of Management, The University of Texas at Austin
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森 一生
Department of Clinical Pharmacy, Oita Medical University
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武山 正治
Department Of Clinical Pharmacy Oita Medical University
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松尾 博文
Department Of Clinical Pharmacy Oita Medical University
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Mori Kazuo
Department Of Clinical Pharmacy Oita Medical University
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