Methanol Extract of Xanthium strumarium L. Possesses Anti-inflammatory and Anti-nociceptive Activities(Pharmacognosy)

概要

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As an attempt to identify bioactive natural products with anti-inflammatory activity, we evaluated the effects of the methanol extract of the semen of Xanthium strumarium L. (MEXS) on lipopolysaccharide (LPS)-induced nitric oxide (NO), prostaglandin E_2 (PGE_2) and tumor necrosis factor-α (TNF-α) production in RAW 264.7 cells. Our data indicate that MEXS is a potent inhibitor of NO, PGE_2 and TNF-α production. Consistent with these findings, the expression levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein and iNOS, COX-2 and TNF-α mRNA were down-regulated in a concentration-dependent manner. Furthermore, MEXS inhibited nuclear factor kappa B (NF-κB) DNA binding activity and the translocation of NF-κB to the nucleus by blocking the degradation of inhibitor of kappa B-α(IκB-α). We further evaluated the anti-inflammatory and anti-nociceptive activities of MEXS in vivo. MEXS (100, 200mg/kg/d, p. o.) reduced acute paw edema induced by carrageenin in rats, and showed analgesic activities in an acetic acid-induced abdominal constriction test and a hot plate test in mice. Thus, our study suggests that the inhibitions of iNOS, COX-2 expression, and TNF-α release by the methanol extract of the semen of Xanthium strumarium L. are achieved by blocking NF-κB activation, and that this is also responsible for its anti-inflammatory effects.
社団法人日本薬学会の論文
2005-01-01