Effect of Ginsenoside Rb_1 on Rat Liver Phosphoproteins Induced by Carbon Tetrachloride

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概要

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• We investigated the effects of ginsenoside Rb_1 (G-Rb_1), a major saponin from Panax ginseng C. A. MEYER, on rat liver protein phosphorylation after intraperitoneal administration of CCl_4 alone or together with G-Rb_1. We found that 118,63,and 34 kDa proteins were prominently phosphorylated in liver homogenates prepared from CCl_4-administered rats, while these protein-phosphorylations were inhibited in the homogenate prepared from the G-Rb_1 plus CCl_4-administration group. When inhibitors of protein kinases were exogenously added to the homogenates from either the CCl_4-administered group or the G-Rb_1 plus CCl_4-administered group, their phosphorylations were inhibited much more by W-7,an inhibitor of Ca^<2+>/calmodulin-dependent protein kinase(CaM-PK), than by H-7,an inhibitor of protein kinase C (C-kinase). Interestingly, only 34 kDa was phosphorylated in homogenates prepared from the corn oil-, G-Rb_1-, and G-Rb_1 plus CCl_4-administered groups by the exogenous addition of sodium fluoride (NaF), an inhibitor of glycogen synthase. Additionally, G-Rb_1 inhibited the Ca^<2+>-accumulation induced by CCl_4 both in liver homogenates and microsomes. The above results imply that G-Rb_1 inhibits the CCl_4-induced protein phosphorylations by modulating CaM-PK rather than C-kinase, and that 34 kDa protein may play a different biological role in cellular environment from 118 and 63 kDa proteins. Therefore, a study in which G-Rb_1 is employed as a modulator of critical CCl_4-induced phenomena ranging from the disturbance of Ca^<2+> concentration to protein phosphorylation may be successfully applicable to investigate the diverse physiological functions of liver.

• 公益社団法人日本薬学会の論文
• 1996-06-15

著者

• Park Hee-juhn

  Division Of Applied Plant Sciences Sangji University

• Park Kyeong-mee

  Department Of Biochemical Pharmacology Korea Ginseng And Tobacco Research Institute

• Song Y‐b

  Department Of Biomedical Pharmacology Korea Ginseng And Tobacco Research Institute

• Rhee Man-hee

  Department Of Biochemical Pharmacology Korea Ginseng And Tobacco Research Institute

• Lee J‐h

  Ewha Womans Univ. Seoul Kor

• PARK Hwa-Jin

  Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering, Inje Uni

• PARK Ki-Hyun

  Department of Biomedical Pharmacology Korea Ginseng and Tobacco Research Institute

• Song Yong-bum

  Department Of Biochemical Pharmacology Korea Ginseng And Tobacco Research Institute

• CHOI Kang-Ju

  Department of Biochemical Pharmacology, Korea Ginseng and Tobacco Research Institute

• LEE Jong-Hwa

  Department of Biochemical Pharmacology, Korea Ginseng and Tobacco Research Institute

• KIM Seok-Chang

  Department of Biochemical Pharmacology, Korea Ginseng and Tobacco Research Institute

• Choi Kang-ju

  Department Of Biochemical Pharmacology Korea Ginseng And Tobacco Research Institute

• Park Hwa

  Department Of Biomedical Laboratory Science College Of Biomedical Science And Engineering And Region

• Park Hwa-jin

  Department Of Biochemical Pharmacology Korea Ginseng And Tobacco Research Institute

• Kim Seok-chang

  Department Of Biochemical Pharmacology Korea Ginseng And Tobacco Research Institute

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