Inhibition of Lipopolysaccharide-Induced Expression of Inducible Nitric Oxide and Cyclooxygenase-2 by Chiisanoside via Suppression of Nuclear Factor-κB Activation in RAW 264.7 Macrophage Cells(Pharmacognosy)

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In the present study, the effects of several triterpenes isolated from the leaves of Acanthopanax chiisanensis (Araliaceae), namely, chiisanoside, isochiisanoside, 22-hydroxychiisanoside and chiisanogenin (the aglycone of chiisanoside) were evaluated on lipopolysaccharide (LPS)-induced nitric oxide (NO) and prostaglandin E_2 (PGE_2) production by the RAW 264.7 macrophage cell line. Of the triterpenes tested, chiisanoside was found to most potently inhibit NO and PGE_2 production. In addition, chiisanoside significantly reduced the release of inflammatory cytokines like TNF-α and IL-1β. Consistent with these observations, the protein and mRNA expression levels of iNOS and COX-2 enzyme were found to be inhibited by chiisanoside in a concentration-dependent manner. Furthermore, chiisanoside inhibited the nuclear factor-κB (NF-κB) activation induced by LPS and this was associated with a reduction in p65 protein in the nucleus and with the phosphorylations of ERK1/2 and JNK MAP kinases. Taken together, our data indicate that the anti-inflammatory properties of chiisanoside might be the result from the inhibition of iNOS, COX-2, TNF-α and IL-1β expression through the down-regulation of NF-κB binding activity.
社団法人日本薬学会の論文
2005-10-01