Inhibition of LPS-Induced iNOS, COX-2 and Cytokines Expression by Poncirin through the NF-κB Inactivation in RAW 264.7 Macrophage Cells(Pharmacognosy)
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概要
- 論文の詳細を見る
We previously reported that poncirin, a flavanone glycoside isolated from the EtOAc extract of the dried immature fruits of Poncirus trifoliata, is an anti-inflammatory compound that inhibits PGE_2 and IL-6 production. The present work was undertaken to investigate the molecular actions of poncirin in RAW 264.7 macrophage cell line. Poncirin reduced lipopolysaccharide (LPS)-induced protein levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) and the mRNA expressions of iNOS, COX-2, tumor necrosis factor-α(TNF-α) and interleukin-6 (IL-6) in a concentration-dependent manner, as determined by Western blotting and RT PCR, respectively. Furthermore, poncirin inhibited the LPS-induced DNA binding activity of nuclear factor-κB (NF-κB). Moreover, this effect was accompanied by a parallel reduction in IκB-α degradation and phosphorylation that in by nuclear translocations of p50 and p65 NF-κB subunits. Taken together, our data indicate that anti-inflammatory properties of poncirin might be the result from the inhibition iNOS, COX-2, TNF-α and IL-6 expression via the down-regulation of NF-κB binding activity.
- 社団法人日本薬学会の論文
- 2007-12-01
著者
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LEE Kyung-Tae
College of Pharmacy, Kyung-Hee University
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Lee Kyung-tae
Department Of Life And Nanopharmaceutical Sciences College Of Pharmacy Kyung Hee University
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Lee Kyung-tae
Coll. Of Medical Sci. Kyung Hee Univ.
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Lee Kyung
College Of Pharmacy Dongdaemun-ku Hoegi-dong Kyung-hee University
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Lee Kyung-tae
Department Of Biochemistry College Of Pharmacy Kyung Hee University
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KIM Jong-Bin
Department of Electronic Engineering, Chosun University
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Lee Kyung-tae
Department Of Pharmacological Biochemistry College Of Pharmacy Kyung Hee University
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Kim Ji-yeon
韓国
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Kim J‐y
Yeungnam Univ. Taegu Kor
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Kim Ji-yeon
Department Of Pharmaceutical Biochemistry And Kyung-hee East-west Pharmaceutical Research Institute
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Han Ah-reum
韓国
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Han Ah-reum
College Of Pharmacy And Center For Cell Signaling & Drug Discovery Research Ewha Womans Universi
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LEE Jun
College of Pharmacy and The Center for Cell Signaling & Drug Discovery Research, Ewha Womans Univers
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SEO Eun-Kyoung
College of Pharmacy and The Center for Cell Signaling & Drug Discovery Research, Ewha Womans Univers
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PARK Eun-Young
Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University
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CHO Woong
Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University
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Lee Jun
Diabetes Research Center Division Of Traditional Korean Medicine (tkm) Integrated Research Korea Ins
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Cho Woong
Department Of Pharmaceutical Biochemistry College Of Pharmacy Kyung-hee University
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Cho Woong
Department Of Pharmaceutical Biochemistry And Kyung-hee East-west Pharmaceutical Research Institute
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Kim Jong-bin
Coll. Of Pharmacy Kyung-hee Univ.
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Kim Jong-pyung
Laboratory Of Antioxidants Korea Research Institute Of Bioscience And Biotechnology
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Park Eun-young
Department Of Pharmaceutical Biochemistry College Of Pharmacy Kyung-hee University
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Park Eun-young
Department Of Pediatrics Hallym University College Of Medicine
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Lee Kyung
Department Of Biochemistry College Of Pharmacy Kyung-hee University
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Kim Jin
Diabetic Complications Res. Center Div. Of Traditional Korean Medicine Integrated Res. Korea Inst. O
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Seo Eun-kyoung
Natural Product Chemistry Laboratory College Of Pharmacy And The Center For Cell Signaling & Dru
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Lee Jun
韓国
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Seo Eun
Natural Product Chemistry Laboratory College Of Pharmacy & The Center For Cell Signaling And Dru
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Lee Jun
College Of Pharmacy Aging Tissue Bank Pusan National University
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Lee Jong-won
College Of Pharmacy Ewha Womans University
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Kim Jong-bin
Department Of Electronic Engineering Chosun University
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Lee Jun
College Of Agriculture And Life Sciences Seoul National University
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Han Ah-reum
Dep. Of Physiology Coll. Of Oriental Medicine Kyung Hee Univ.
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Kim Ji-yeon
Department Of Pharmaceutical Biochemistry College Of Pharmacy Kyung-hee University
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Lee Jun
College of Agriculture and Life Science, Seoul National University, Seoul 151-742, Korea
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