Styraxoside A Isolated from the Stem Bark of Styrax japonica Inhibits Lipopolysaccharide-Induced Expression of Inducible Nitric Oxide Synthase and Cyclooxygenase-2 in RAW 264.7 Cells by Suppressing Nuclear Factor-kappa B Activation(Pharmacognosy)

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In the present study, the effects of terpenes (styraxosides A and B) and lignans (egonol, masutakeside I, and styraxlignolide A) isolated from the stem bark of Styrax japonica SIEB. et ZUCC. (styracaceae) were evaluated on lipopolysaccharide (LPS)-induced nitric oxide (NO) and prostaglandin E_2 (PGE_2) production by the RAW 264.7 macrophage cell line. Of the tested compounds, styraxoside A was found to most potently inhibit the productions of NO and PGE_2, and also significantly reduced the release of tumor necrosis factor-α (TNF-α) and interleukin-1β(IL-1β). Consistent with these observations, the protein expression levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) and the mRNA expression levels of iNOS, COX-2, TNF-α and IL-1β were found to be inhibited by styraxoside A in a concentration-dependent manner. Furthermore, styraxoside A inhibited the LPS-induced DNA binding activity of nuclear factor-κB (NF-κB). Taken together, our data indicate that styraxoside A inhibits LPS-induced iNOS, COX-2, TNF-α, and IL-1β expressions through the down-regulation of NF-κB-DNA binding activity.
公益社団法人日本薬学会の論文
2007-01-01