Inhibition of Human Low Density Lipoprotein and High Density Lipoprotein Oxidation by Oligostilbenes from Rhubarb(Pharmacognosy)

概要

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The objective of the present study was to elucidate the beneficial properties of ampelopsine B (1) and ε-Viniferin (2), two oligostilbenes isolated from rhubarb, toward cardiovascular disease by protecting human lipoproteins against lipid peroxidation. In low density lipoprotein (LDL) oxidation, both 1 and 2 exert an inhibitory activity against Cu^<2+>-,2,2'-azobis-(2-amidinopropane) hydrochloride (AAPH)-induced, as exhibited by prolongation of lag time from 52 to 118 and 136min, respectively, and also increasing the lag time 38 to 105 and 128min in high density lipoprotein (HDL) oxidation for 1 and 2, respectively, at the concentration of 3.0μM. In generation of thiobarbituric acid reactive subtances (TBARS), compounds 1 and 2 inhibited LDL oxidation mediated by either catalytic Cu^<2+> or thermo-labile radical initiator (AAPH) in a dose-dependent manner with IC_<50> values of 3.6 and 6.0μM for 1, and 1.7 and 3.2μM for 2, respectively. In addition, compounds 1-2 also showed strong ability to protect HDL oxidation induced by both Cu^<2+> and AAPH with low IC_<50> values. The results suggest that oligostilbenes 1-2 may have a role in preventing lipoprotein oxidation.
公益社団法人日本薬学会の論文
2008-09-01