Identification of Panax Species in the Herbal Medicine Preparations Using Gradient PCR Method(Pharmacognosy)

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概要

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• In order to identify the existence of Panax species in herbal medicine preparations, the Ginseng specific marker primer was selected and created based on the sequence of Korean ginseng DNA fragment, 359bp. The gradient PCR was performed on 40 types of the herbal medicines including the 7 types of Araliaceae that are in the same family with the Panax ginseng using the created Ginseng maker primer. As result, Panax notoginseng (Chinese), Panax japonicus (Japanese) and Panax quinquefolius (American), along with Panax ginseng (Korean) were the only ones amplified. However, in the case of Atractylodes lancea, one of the herbal medicines not categorized as Panax species, the DNA was prominently amplified by the Ginseng marker primer. The sequence of the amplified DNA of Atractylodes lancea was identified, resulting in enabling the differentiation from the Panax species by the Restriction Fragment Length Polymorphisms (RFLP) method. In addition, the results of the gradient PCR performed on the herbal medicine preparations that consists of Panax ginseng showed that 290bp size of the original DNA fragments of Panax ginseng was amplified on the herbal medicine preparations containing Panax ginseng. Therefore, these results suggest a possibility of creating a new testing method for identifying specific herb medicines using the gradient PCR, a molecular biological method not only on Panax ginseng, but also on other herbal medicines and herbal medicine preparations.

• 公益社団法人日本薬学会の論文
• 2005-04-01

著者

• KIM Hong

  College of Medicine, Kohwang Medical Research Institute, Kyung Hee University

• Cho Myung

  Department Of Biology Konkuk University

• Kim Do

  Korea Foood & Drug Administration

• Cho Jung

  韓国

• SHIM Young

  College of Pharmacy, Chung Ang University

• PARK Chan

  Korea Foood & Drug Administration

• CHO Jung

  Korea Foood & Drug Administration

• Shim Young

  College Of Pharmacy Chung Ang University

• Kim Hong

  College Of Pharmacy Chungnam National University

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