Inhibitory Effects of Nardostachin on Nitric Oxide, Prostaglandin E_2, and Tumor Necrosis Factor-α Production in Lipopolysaccharid Activated Macrophages(Biochemistry/Molecular Biology)

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Nardostachin, which is an iridoid isolated from Patrinia saniculaefolia, was examined by assessing its effect on the production of tumor necrosis factor-α (TNF-α) and expression of 2 enzymes, inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), in lipopolysaccharide (LPS)-stimulated Raw264.7 macrophages. This compound consistently inhibited the production of nitric oxide (NO) and TNF-α production in a dose-dependent manner, with respective IC_<50> values of 12.3 and 16.2 μM. The decrease in quantity of NO products was accompanied by a decrease in the iNOS protein level, as assessed by Western blotting probed with specific anti-iNOS antibodies. In addition, this compound also reduced the COX-2 protein expression level and the attendant PGE_2 production in LPS-stimulated macrophages. These results suggest that nardostachin may be useful for inhibiting the production of inflammatory mediators such as TNF-α, NO and PGE_2 in inflammatory diseases.
2003-10-01