Identification and Characterization of Antioxidants from Sophora flavescens(Pharmacognosy)

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概要

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• The objectives of this study were to investigate the radical-scavenging activity and protective potential of Sophora flavescens from oxidative damage by the radical generator 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH) in renal epithelial LLC-PK_1 cells and to identify the active components using the bioassay-linked fractionation method. The MeOH extract and fractions of CH_2Cl_2, BuOH, and H_2O from S. flavescens showed 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging effects in a dose-dependent manner (p<0.01), whereas only the BuOH and CH_2Cl_2 fractions showed protective effects against LLC-PK_1 cellular damage induced by AAPH in a dose-dependent manner (p<0.01). In particular, the BuOH fraction had the most effective (p<0.05) antioxidative capacity. Employing a bioassay-linked HPLC/MS method, the active constituents from the BuOH fraction of S. flavescens were isolated and characterized as sophoraflavanone G and kurarinone with potent antioxidant effects against the DPPH radical, with IC_<50> values of 5.26 and 7.73μg/ml, respectively. Moreover, the compounds dose dependently recovered cell viability decreased by AAPH treatment (p<0.01), suggesting their protective roles against cellular oxidative damage. The results of this study suggest that S. flavescens has excellent antioxidative and kidney-protective potential and that flavonoids from S. flavescens, i.e., sophoraflavanone G and kurarinone, are the active constituents.

• 公益社団法人日本薬学会の論文
• 2006-09-01

著者

• Kim Sung

  Department of Bioengineering and Technology, College of Engineering, Kangwon National University

• Kim Hyun

  College Of Pharmacy Kangwon National University

• Park Jeong

  Res. Inst. Of Pharmaceutical Sciences Coll. Of Pharmacy Seoul National Univ.

• PARK Jeong

  College of Pharmacy, Seoul National University

• PIAO Xiang

  College of Pharmacy, Seoul National University

• Park Jeong

  College Of Pharmacy And Research Institute Of Pharmaceutical Sciences Seoul National University

• Cai Shao-qing

  School Of Pharmaceutical Sciences Peking University

• Piao Xiang-lan

  School Of Pharmaceutical Sciences Peking University

• Piao Xiang

  College Of Animal Science And Technology China Agriculture University Mafic

• Kim Sung

  Department Of Anatomy

• Cai Shao-qing

  中華人民共和国

• Kim Hyun

  富山大学和漢医薬学総合研究所

• Kim Sung

  Department Of Animal And Food Sciences Texas Tech University

• Kim Hyun

  College Of Pharmacy And Research Institute Of Drug Development Chonnam National University

• Kim Hyun

  College Of Electrical & Computer Engineering Chungbuk National University

• Kim Sung

  Department of Advanced Materials Engineering for Information and Electronics, Kyung Hee University, Yongin, Gyeonggi 446-701, Republic of Korea

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