Process development for mycelial growth and polysaccharide production in Tricholoma matsutake liquid culture(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
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概要
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In this study, the effects of agitation and aeration on mycelial growth and exo-polysaccharide production were examined in batch cultures of Tricholoma matsutake. Agitation was varied from 100 to 300rpm and aeration was varied from 0.5 to 1.5vvm. Mycelial growth was 21.87g/l at 150rpm, and exo-polysaccharide production was 8.79g/l at 1.5vvm. When we analyzed the polysaccharide extractions from the cultured mycelium and the culture broth of T. matsutake, 1.4g of crude polysaccharide was found per 100g of dried weight in the cultured mycelium, and 1.47g/l of polysaccharides was found in the culture broth. In addition, the amounts of β-Glucan in the soluble polysaccharide fractions of the cultured mycelium and culture broth were 75.4% and 83.6%, respectively. The cultured mycelium and the culture broth contained a higher amount of β-Glucan than that of the fruiting body.
著者
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LEE Jong
Department of Polymer Science and Engineering, Kumoh National Institute of Technology
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Kim Sung
Department of Bioengineering and Technology, College of Engineering, Kangwon National University
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Cho Jae
Department of Biomaterials Engineering, College of Bioscience and Biotechnology, Kangwon National Un
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Kim Young
Korea Food Research Institute
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Hong Eock
Department of Bioengineering and Technology, College of Engineering, Kangwon National University
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Hong Eock
Department Of Bioengineering And Technology College Of Engineering Kangwon National University
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Kim Sung
Department Of Bioengineering And Technology College Of Engineering Kangwon National University
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Kim Young
Korea And College Of Pharmacy Chungbuk National University
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Kim Sung
Department Of Anatomy
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Cho Jae
Department Of Biomaterials Engineering College Of Bioscience And Biotechnology Kangwon National Univ
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Lee Jong
Department Of Bioengineering And Technology College Of Engineering Kangwon National University
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Lee Jong
Department Of Anesthesiology And Pain Medicine Dong-a University Hospital
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Lee Jong
Depart. Of Oncology Yonsei Univ. Wonju College Of Medicine
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Kim Sung
Department of Advanced Materials Engineering for Information and Electronics, Kyung Hee University, Yongin, Gyeonggi 446-701, Republic of Korea
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