Protective Effects of Broccoli (Brassica oleracea) and Its Active Components against Radical-Induced Oxidative Damage

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概要

• 論文の詳細を見る

• The radical scavenging effect and protective potential from oxidative damage by radical generator, 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH), in renal epithelial LLC-PK_1 cell of broccoli (Brassica oleracea) were investigated and identified the active components under the bioassay-linked fractionation method. The MeOH extract, and fractions of CH_2Cl_2, BuOH and H_2O from broccoli showed the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging effect in a dose-dependent manner. In addition, they exerted the protective effect against LLC-PK_1 cellular damage induced by AAPH dose-dependently. In particular, the BuOH fraction was evaluated as the most active fraction, indicating that the BuOH fraction contains the active components with antioxidative capacity. Employing a bioassay-linked fractionation method, the active principles were isolated and characterized as 1,2-disinapoylgentiobiose and 1-sinapoyl-2-feruloylgentiobiose from the BuOH fraction. These two compounds from broccoli displayed potent antioxidant effects against the DPPH radical, showing the IC_<50> values of 5.18 and 7.52μg/mL, respectively. Moreover, the compounds significantly and dose-dependently recovered cell viability lowered by AAPH treatment, suggesting the protective roles from cellular oxidative damage. The present study suggests that broccoli has excellent antioxidative potential and the hydroxycinamic acid esters from broccoli, 1,2-disinapoylgentiobiose and 1-sinapoyl-2-feruloylgentiobiose, are considered as the active components with antioxidative effect.

• 日本ビタミン学会の論文

著者

• Yokozawa Takako

  Institute of Natural Medicine, University of Toyama

• CHO Eun

  Department of Pathology, Samsung Medical Center, Sungkyunkwan University School of Medicine

• Kim Hyun

  College Of Pharmacy Kangwon National University

• Yokozawa Takako

  Inst. Of Natural Medicine Univ. Of Toyama

• Yoshikawa T

  Institute Of Natural Medicine University Of Toyama

• LEE Young

  Institute of Natural Medicine, University of Toyama

• PIAO Xiang

  College of Pharmacy, Seoul National University

• LEE Young

  Department of Food Science and Nutrition, Pusan National University

• Kim Hyun

  Dep. Of Food Sci. Jinju National Univ.

• Cho Eun

  Department Of Food Science And Nutrition Pusan National University

• Cho E

  Institute Of Natural Medicine Toyama Medical And Pharmaceutical University

• Piao Xiang

  College Of Pharmacy Seoul National University

• Piao Xiang

  College Of Animal Science And Technology China Agriculture University Mafic

• Yokozawa Takako

  Institute Of Natural Medicine Toyama Medical And Pharmaceutical University

• CHO Eun

  富山医科薬科大学和漢薬研究所

• Lee Young

  Department Of Aerospace Information Engineering The Konkuk University

• Cho Eun

  Department Of Dermatology St Vincent Hospital College Of Medicine The Catholic University Of Korea

• Lee Young

  Institute Of Natural Medicine University Of Toyama

• Kim Hyun

  Univ. Toyama Toyama Jpn

• 横澤 隆子

  Inst. Of Natural Medicine Univ. Of Toyama

• Kim H

  Univ. Toyama Toyama Jpn

• Kim Hyun

  College Of Pharmacy And Research Institute Of Drug Development Chonnam National University

• Cho Eun

  Department Of Chemistry Yeungnam University

• Kim Hyun

  College Of Electrical & Computer Engineering Chungbuk National University

• CHO Eun

  Department of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology

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