Expression Patterns of Plasma Proteins in Spontaneously Diabetic Rats after Oral Administration of a Kampo Medicine, Hachimi-jio-gan, Using SELDI ProteinChip Platform(Pharmacognosy)
スポンサーリンク
概要
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We investigated the changes (increase or decrease in peak intensity) in the expression of plasma proteins in spontaneously diabetic WBN/Kob rats that were with complicated diabetic nephropathy, to determine multiple biomarkers in the plasma of diabetic rats. The present study using surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) demonstrated that six peaks at mass/charge ratios (m/z) of 4678, 4732, 4808, 9058, 9323, and 9465, among approximately 80 peaks per spectrum in the 2000-10000 Da mass range, had increased peak intensities with the development or progression of diabetic nephropathy in plasma of spontaneously diabetic WBN/Kob rats as compared with those of normal Wistar rats. Administration of the Kampo medicine Hachimi-jio-gan was effective at reducing the expression of diabetic nephropathy but not at reducing blood glucose levels. It also improved the increased levels of these plasma proteins. Other biomarker peaks at m/z 5067, 5279, 7598, and 7917 were not affected by Hachimi-jio-gan administration. Further study will be needed to identify these positive biomarkers and to evaluate the relationship between the efficacy and expression patterns of the plasma proteins in greater detail. The expression patterns of proteins and molecular-related ions revealed that several proteins in plasma may be involved in the development and/or progression of diabetic nephropathy in WBN/Kob rats and the efficacy of Hachimi-jio-gan. This study using ProteinChip technology may provide a useful basis in the search for multiple biomarkers in plasma for the diagnosis of disease and therapeutic evaluation of Kampo medicines.
- 社団法人日本薬学会の論文
- 2005-06-01
著者
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横澤 隆子
富山医科薬科大学和漢薬研究所
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SAIKI Ikuo
Department of Pathogenic Biochemistry, Institute of Natural Medicine, Toyama Medical and Pharmaceuti
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中川 孝子
富山大学和漢医薬学総合研究所漢方診断学部門
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Saiki Ikuo
Div. Of Pathogenic Biochemistry Inst. Of Natural Medicine Univ. Of Toyama
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Yokozawa Takako
Univ. Toyama Toyama Jpn
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Yoshikawa T
Institute Of Natural Medicine University Of Toyama
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KOIZUMI Keiichi
Department of Biopharmaceutics, School of Pharmaceutical Sciences, Osaka University
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GOTO Hirozo
Department of Kampo Diagnostics, Institute of Natural Medicine, Toyama Medical and Pharmaceutical Un
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Shimada Y
Dept. Of Japanese Oriental Medicine Faculty Of Medicine University Of Toyama
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NAKAGAWA Takako
Department of Kampo Diagnostics, Institute of Natural Medicine, University of Toyama
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KIGA Chizuru
Department of Pathogenic Biochemistry, Toyama Medical and Pharmaceutical University
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SAKURAI Hiroaki
Department of Pathogenic Biochemistry, Toyama Medical and Pharmaceutical University
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SHIBAGAKI Yukari
Bioinformatics Division, INTEC Web and Genome Informatics Corporation
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OGAWA Kazuo
Medical Evaluation Laboratory, Research Division, Tsumura and Co.
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Goto Hirozo
Toyama Medical and Pharmaceutical University
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横澤 隆子
富山大学和漢医薬学総合研究所
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Goto H
Department Of Japanese Oriental Medicine Faculty Of Medicine University Of Toyama
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Goto Hirozo
富山大学和漢医薬学総合研究所 病態生化学
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Goto Hirozo
富山大学 大学院医学薬学研究部和漢診療学講座
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Goto H
富山医科薬科大学和漢薬研究所漢方診断学部門
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Goto Hirozo
Department Of Kampo Diagnostics Institute Of Natural Medicine University Of Toyama
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Shimada Y
Tokyo Inst. Technol. Yokohama Jpn
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Shimada Y
Institute Of Multidisciplinary Research For Advanced Materials Tohoku University
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Shimada Yuji
Microsystem Research Center Precision And Intelligence Laboratory Tokyo Institute Of Technology
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Shimada Y
Tohoku Univ. Sendai Jpn
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島田 洋蔵
産業技術総合研究所
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Goto Hirozo
Department Of Japanese Oriental Medicine Faculty Of Medicine University Of Toyama
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嶋田 豊
富山医科薬科大学医学部和漢診療学
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Sakurai Hiroaki
Div. Of Pathogenic Biochemistry Inst. Of Natural Medicine Univ. Of Toyama
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横澤 隆子
富山医科薬科大学和漢薬研究所細胞資源工学部門
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後藤 博三
富山医科薬科大学和漢薬研究所漢方診断学部門
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Goto Hirozo
Department Of Japanese Oriental Medicine Faculty Of Medicine University Of Toyama : 21st Century Coe
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Kiga Chizuru
富山大学和漢医薬学総合研究所 病態生化学
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Kiga Chizuru
Department Of Pathogenic Biochemistry Toyama Medical And Pharmaceutical University:toyama New Indust
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Nakagawa Takako
Department Of Kampo Diagnostics Institute Of Natural Medicine University Of Toyama
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Nakagawa Takako
Department Of Japanese Oriental Medicine Toyama Medical And Pharmaceutical University
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Nakagawa Takako
Department Of Anesthesia Ntt West Hospital
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Shibagaki Yukari
富山大学和漢医薬学総合研究所
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Koizumi Keiichi
Department Of Pathogenic Biochemistry Toyama Medical And Pharmaceutical University
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島田 洋蔵
Dept. Of Japanese Oriental Medicine Faculty Of Medicine University Of Toyama
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Saiki Ikuo
Department Of Pathogenic Biochemistry Institute Of Natural Medicine Toyama Medical And Pharmaceutica
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Shimada Yutaka
21^<st> Century Coe Program Toyama Medical And Pharmaceutical University
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Shibagaki Yukari
Bioinformatics Division Intec Web And Genome Informatics Corporation
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Saiki Ikuo
Department Of Pathogenic Biochemistry Institute Of Natural Medicine Toyama Medical And Pharmaceutica
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Sakurai Hiroaki
Department Of Bioscience Institute Of Natural Medicine Toyama University
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Saiki Ikuo
Department Of Bioscience Institute Of Natural Medicine Toyama University
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