Protective Effects of Broccoli (Brassica oleracea) against Oxidative Damage in Vitro and in Vivo
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概要
- 論文の詳細を見る
The antioxidative effect and protective potential against diabetes of the broccoli flower were investigated both in vitro and in a diabetic rat model. Among fractions of MeOH, CH_2Cl_2, BuOH, and H_2O, the BuOH fraction exerted the strongest inhibitory activities on 1, 1-diphenyl-2-picrylhydrazyl radical, radical-induced protein oxidation, and nitric oxide generation by sodium nitroprusside. The in vitro results suggest that the BuOH fraction from the broccoli flower has a protective potential against oxidative stress. The rat model with diabetes induced by streptozotocin was employed to evaluate the protective effect of the BuOH fraction in vivo. Diabetic rats showed reduced body weight gain and heavier kidney and liver weights than normal rats, while oral administration of the BuOH fraction at an oral dose of 100 or 200mg/kg body weight/d for 20 d attenuated the physiological changes induced by diabetes. In addition, oral administration of the BuOH fraction to diabetic rats led to significant decreases in serum glucose and glycosylated protein, while it resulted in the increase of serum albumin, implying that the BuOH fraction improves the abnormal metabolism of glucose and protein that leads to oxidative stress. Moreover, it significantly reduced thiobarbituric acid-reactive substance levels in serum, hepatic and renal mitochondria. This suggests that the BuOH fraction would alleviate the oxidative stress associated with diabetes through the inhibition of lipid peroxidation. The present study demonstrates that the BuOH fraction has an antioxidative effect in vitro and it protects against oxidative stress induced by diabetes in an in vivo model.
- 日本ビタミン学会の論文
著者
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Yokozawa Takako
Institute of Natural Medicine, University of Toyama
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CHO Eun
Department of Pathology, Samsung Medical Center, Sungkyunkwan University School of Medicine
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Yokozawa Takako
Inst. Of Natural Medicine Univ. Of Toyama
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Yoshikawa T
Institute Of Natural Medicine University Of Toyama
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LEE Young
Institute of Natural Medicine, University of Toyama
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Yoo Hye
Institute of Natural Medicine, University of Toyama
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Cho Eun
Department Of Food Science And Nutrition Pusan National University
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Cho E
Institute Of Natural Medicine Toyama Medical And Pharmaceutical University
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Yoo Hye
Institute Of Natural Medicine University Of Toyama
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Yokozawa Takako
Institute Of Natural Medicine Toyama Medical And Pharmaceutical University
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CHO Eun
富山医科薬科大学和漢薬研究所
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Lee Young
Institute For Protein Research Osaka University And Crest Japan Science And Technology Cooperation
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Cho Eun
Department Of Dermatology St Vincent Hospital College Of Medicine The Catholic University Of Korea
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Lee Young
Institute Of Natural Medicine University Of Toyama
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横澤 隆子
Inst. Of Natural Medicine Univ. Of Toyama
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Cho Eun
Department Of Chemistry Yeungnam University
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Lee Young
Institute For Geomatics Korean Association Of Surveying & Mapping
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CHO Eun
Department of Animal Science and Biotechnology, Gyeongnam National University of Science and Technology
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