Effect of Dexamethasone on STAT6-Dependent Ym1/2 Expression in Vivo and in Vitro(Biochemistry)
スポンサーリンク
概要
- 論文の詳細を見る
Ym1 and Ym2 (Ym1/2) are chitinase-like proteins and we reported previously that IL-4 induced Ym1/2 in mouse bone marrow-derived mast cells. In the present study, ovalbumin-induced asthmatic mice were used to investigate the effect of glucocorticoids on Ym1/2 expression. Ym1/2 were highly induced in bronchoalveolar lavage fluid (BALF) and the lung. Ym1/2 expression was completely inhibited by dexamethasone (Dex) in BALF and weakly inhibited in the lung. Primary cultured macrophages were used to investigate the inhibition of Ym1/2 expression at the cellular level. Although Dex pretreatment inhibited the Ym1/2 expression level in an animal model, it did not reduce IL-4 induction of Ym1/2 expression in vitro. Next, we tested whether Dex blocks IL-4 induced STAT6 signaling and found that it had no inhibitory effect on the phosphorylation level of STAT6 in macrophages. The luciferase reporter assay also revealed that Dex did not inhibit IL-4 induction of Ym1/2 promoter activity. These results indicate that the inhibitory effect of Dex on Ym1/2 protein expression in the murine model of asthma does not involve the STAT6 signaling pathway.
- 2008-09-01
著者
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LEE Eunkyung
College of Pharmacy, Yeungnam University
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JIN Meihua
College of Pharmacy, Yeungnam University
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QUAN Zhejiu
College of Pharmacy, Yeungnam University
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HWANG Nam
College of Pharmacy, Yeungnam University
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LIN Chang
College of Pharmacy, Yeungnam University
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KANG Sanggu
School of Biotechnology, Yeungnam University
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MOON Tae
Department of Medicine, University of Alberta
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CHANG Hyeun
College of Pharmacy, Yeungnam University
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Lin Chang
College Of Pharmacy Yeungnam University
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Lin Chang
韓国
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Chang Hyeun
College Of Pharmacy Yeungnam University
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Kang Sam
韓国
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Moon Tae
韓国
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Moon T
Pulmonary Research Group Department Of Medicine University Of Alberta
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Kang Sam
Natural Products Research Institute And College Of Pharmacy Seoul National University
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Moon Tae
Pulmonary Research Group Department Of Medicine University Of Alberta
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Chang Hyeun
Coll. Of Pharmacy Yeungnam Univ.
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Moon Tae
Department Of Agricultural Biotechnology And Center For Agricultural Biomaterials Seoul National Uni
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Kang S
Natural Products Research Institute And College Of Pharmacy Seoul National University
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Lee E
Yeungnam Univ. Gyongsan Kor
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Hwang Nam
College Of Pharmacy Yeungnam University
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Quan Zhejiu
College Of Pharmacy Yeungnam University
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Chang H
College Of Pharmacy Yeungnam University
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Jin Mei
College Of Pharmacy Yeungnam University
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Lee Eunkyung
College Of Pharmacy Yeungnam University
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