Induction of Apoptosis by Korean Medicine Gagam-whanglyun-haedoktang Through Activation of Caspase-3 in Human Leukemia Cell Line, HL-60 Cells
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概要
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Gagam-whanglyun-haedoktang (GWH) is a newly designed herbal drug formula based on the traditional oriental pharmacological knowledge for the purpose of treating tumorous diseases. Apoptosis is an evolutionarily conserved suicide program residing in cells. In the present study, apoptosis inducing activities of the decocted water extract of GWH were studied. Results of the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that GWH had a strong cytotoxic effect on HL-60 cells. The number of live cells was less than 20% after exposure to 1 mg/ml GWH for 48 h. GWH increased cytotoxicity of HL-60 cells in a dose- and time-dependent manner. The percentage of apoptotic cells by flow cytometric analysis of the DNA-stained cells increased to 28%, 31%, and 37% at 24 h and to 37%, 44%, and 81% at 48 h after treatment with 0.01, 0.1, and 1 mg/ml GWH, respectively. DNA fragmentation also occurred in apoptosis and was characterized by a ladder pattern on agarose gel. In addition, GWH increased the secretion of tumor necrosis factor-α. GWH-induced apoptosis was accompanied by activation of caspase-3. These results suggest that GWH induces activation of caspase-3 and eventually leads to apoptosis.
- 社団法人 日本薬理学会の論文
- 2005-01-20
著者
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Jeong Hyun-ja
Coll. Of Oriental Medicine And Inst. Of Oriental Medicine Kyung Hee Univ.
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Jeong Hyun-ja
Dep. Of Anatomy School Of Medicine Chonbuk National Univ.
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Kim Hyung-Min
Department of Pharmacology, College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee
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Choi Jong-won
韓国
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Kim You-ah
Div. Of Marine Environment & Bioscience Korea Maritime Univ.
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Hong Seung-heon
Vestibulocochlear Research Center Of Wonkwang University
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Hong S‐h
韓国
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Hong Seung-heon
韓国
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Hong Seung-heon
Department Of Oriental Pharmacy College Of Pharmacy Wonkwang University
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Na Ho-jeong
韓国
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Kim Hyung-min
韓国
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WON Jong-Heon
Department of Biochemistry, College of Pharmacy, Kyung-Hee University
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Jeong H‐j
Dep. Of Oriental Pharmacy Wonkwang Univ.
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Kim Yun-kyung
Department Of Oriental Pharmacy College Of Pharmacy Wonkwang University
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Kim You-ah
韓国
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Kim Y‐k
Wonkwang Univ. Jeonbuk Kor
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WON Jin-Hee
College of Oriental Medicine, Wonkwang University
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Jung H‐s
Division In Anatomy And Developmental Biology Department Of Oral Biology College Of Dentistry Yonsei
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Kim H‐m
Kyung Hee Univ. Seoul Kor
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Kim Hyung
College Of Oriental Medicine Kyung Hee University
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Kim Hyung-min
Department Of Pharmacology College Of Oriental Medicine Kyung Hee University
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Won J‐h
Department Of Biochemistry College Of Pharmacy Kyung-hee University
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Kim Jin-man
Department Of Food And Nutrition College Of Health Sciences Korea University
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Jung Hyuk-sang
Department Of Anatomy College Of Oriental Medicine Kyung Hee University
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Na Ho‐jeong
College Of Pharmacy Wonkwang University
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Won Jin-hee
College Of Oriental Medicine Wonkwang University
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KIM Joon-Bae
Department of Pharmacology, College of Oriental Medicine, Kyung Hee University
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KOO Hyun-Na
Department of Pharmacology, College of Oriental Medicine, Kyung Hee University
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JOENG Hyun-Ja
Department of Pharmacology, College of Oriental Medicine, Kyung Hee University
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LYU Yeoung-Su
College of Oriental Medicine, Wonkwang University
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PARK Sang-Goo
College of Oriental Medicine, Wonkwang University
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Kim Hyung‐min
Dep. Of Anatomy School Of Medicine Chonbuk National Univ.
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Kim Hyung-ryong
Dep. Of Dental Pharmacology And Wonkwang Biomaterial Implant Res. Inst. School Of Dentistry Wonkwang
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Kim H.
韓国
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Kim H‐m
Yonsei Univ. School Of Medicine
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Kim Joong-man
Department Of Pharmacology College Of Oriental Medicine Kyung Hee University
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Lyu Yeoung-su
College Of Oriental Medicine Wonkwang University
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Park Sang-goo
College Of Oriental Medicine Wonkwang University
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Won Jong-heon
韓国
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Na Ho‐jeong
韓国
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So Hong-seob
韓国
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Jung H‐a
Coll. Of Oriental Medicine And Inst. Of Oriental Medicine Kyung Hee Univ.
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Na Ho‐jeong
Dep. Of Pharmacology Coll. Of Oriental Medicine Inst. Of Oriental Medicine Kyung Hee Univ.
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Kim Yun-kyung
Department Of Biological Science Sookmyung Women's University
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Kim Hong‐joon
Department Of Veterinary Medicine College Of Life Sciences Research Institute For Subtropical Agricu
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Joeng Hyun-ja
Department Of Pharmacology College Of Oriental Medicine Kyung Hee University
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Shin H‐y
Kyung Hee Univ. Seoul Kor
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