Expression and Function of CD8 α/β Chains on Rat and Human Mast Cells (Miscellaneous)
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概要
- 論文の詳細を見る
The expression and functional role of CD8 glycoprotein, a marker of cytotoxic/suppressor T lymphocytes and NK cells, were not studied on freshly isolated connective tissue type rat peritoneal mast cells, a rat mucosal type mast cell line (RBL 2H3), or human mast cell line (HMC-1). We used the reverse transcription-polymerase chain reaction (RT-PCR) and Western blot analysis, immunohistochemistry and enzyme-linked immunosorbent assay. Results : RT-PCR and Western blot analysis identified the presence of CD8 α/β chains on the mast cells, and immunohistochemistry confirmed CD8α expression on rat or human mast cells. Functional studies demonstrated that stimulation of CD8 α/β chains on rat mast cells induced the secretion of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), which are regarded as important mediators during infection. However, costimulation with stem cell factor had no effect on CD8-induced mediator secretion. Our findings demonstrate novel biological roles of CD8 molecules in mast cells.
- 公益社団法人日本薬学会の論文
- 2004-03-01
著者
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Kim Hyung-Min
Department of Pharmacology, College of Oriental Medicine, Institute of Oriental Medicine, Kyung Hee
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LEE Hye-Jung
Graduate School of East-West Medical Science, Kyung Hee University
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KIM Sung-Hoon
Graduate School of East-West Medical Science, Kyunghee University
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KIM Mi-Sun
Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University
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Lee Hye-jung
Graduate School Of East-west Medical Science Kyunghee University
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Kim Hyung-min
Department Of Pharmacology College Of Oriental Medicine Kyung Hee University
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Kim Mi-sun
Department Of Pharmacology College Of Oriental Medicine Kyung Hee University
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Kim Sung-hoon
Graduate School Of East-west Medical Science Kyunghee University
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Kim Mi-sun
Department Of Dermatology Seoul National University College Of Medicine
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