ローヤルゼリー中の残留キノロン剤分析のためのイムノアフィニティークロマトグラフィーを用いる前処理法

概要

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A sample preparation method was developed for the determination of qunolones in royal jerry (RJ) using immunoaffinity chromatography. For this purpose, an immunoaffinity column was prepared by packing a quinolone-specific monoclonal antibody immobilized to agarose resin. An RJ sample diluted with phosphate buffer was centrifuged. Then the supernatant was applied to the column. After the column was washed, quinolones were eluted with glycine-HCl. Quinolones in the eluate were determined by HPLC with fluorescence detection. No interfering peak was found on the chromatogram of the RJ sample. The recoveries of quinolones from the samples were over 75% at fortification levels of 25 ng/g for norfloxacin, 10 ng/g for danofloxacin and 50 ng/g for enrofloxacin and ciprofloxacin. This sample-preprocessing method using immunoaffinity chromatography was found to be effective and suitable for determining residual quinolones in RJ.
社団法人　日本分析化学会の論文
2009-12-05