紫外部吸収検出器付高速液体クロマトグラフィーによる食品添加物アミノ酸の光学異性体分析

概要

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Amino acids are authorized food additives commonly used as seasonings, dietary supplements and quality-retaining agents. We have developed a chromatographic method of analyzing racemic acids using HPLC with UV detection. The HPLC conditions were as follows : column 1, SUMICHIRAL OA-5000 (particle type, 5 μm, 4.6 mm i.d. × 150 mm) : column 2, SUMICHIRAL OA-5000 (monolithic type, 4.6 mm i.d. × 100 mm) : mobile phase A, 0.5 mM〜2 mM CuSO_4・5H_2O solution : mobile phase B, 2 mM CuSO_4・5H_2O in water and isopropyl alcohol solution : flow rate, 0.8 mL/min or 1.0 mL/min : detection, UV 254 nm. We separated each racemate of Asn, Cystine and Ser by using the combination of particle type column and monolithic type column. The other racemates were separated by using particle type column alone. The detection ratio of D/L of each amino acid, the limited lower concentration of D-enantiomer for 100 μg/mL of L-enantiomer in simultaneous analysis, were analyzed. The ratios of Glu, Asn, Cys, Thr and Trp were 0.1, and that of the other amino acids were under 0.01. The quantitative limit of D/L-Cys were 10 μg/mL, and the limits of the other amino acids were under 1 μg/mL.
2008-08-20