高速液体クロマトグラフィーによる鶏肉, 豚肉中のバージニアマイシンの定量

元データ 1989-02-28 社団法人日本薬学会

概要

A simple and rapid determination of virginiamycin (VGM) in chicken and swine tissues by quantitation of virginiamycin M_1 (M_1), which is a main component of VGM, was developed. M_1 was extracted from the tissues with a mixture of methanol-3% phosphotungstic acid (7 : 3), and the extract was cleaned up by liquid-liquid partitioning with chloroform. The chloroform extract was further cleaned up on a Sep-Pak silica cartridge using chloroform-methanol (97 : 3) as an eluent. M_1 was determined by reversed phase HPLC which was performed on a Kaseisorb LC-ODS-300-5 column with acetonitrile-water (35 : 65) solution as a mobile phase, and M_1 was monitored with UV detection at 235 nm. The caliblation curve was rectilinear in the range from 0.5 to 10.0μg/ml. The average recoveries were 93.6% and 95.2% for VGM added to chicken and swine tissues at the level of 1.0μg/g, respectively. A good correlation obtained between the HPLC method and the bioassay method shows that the present method will be applicable to determine VGM with the detection limit of 0.05μg/g.

著者

堀江 正一 埼玉県衛生研究所
中澤 裕之 星薬科大学薬品分析化学教室
斉藤 貢一 星薬科大学薬品分析化学教室
星野 庸二 埼玉県衛生研究所
堀江 正一 大妻女子大学
藤田 昌彦 帝国臓器製薬(株)
中澤 裕之 埼玉県衛生研究所
藤田 昌彦 国立公衆衛生院衛生薬学部
斉藤 貢一 埼玉県衛生研究所
能勢 憲英 埼玉県衛生研究所
中澤 裕之 国立公衆衛生院
中澤 裕之 愛知県衛生研究所
藤田 昌彦 国立公衆衛生院

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