高速液体クロマトグラフィーによる養殖魚中の水産用医薬品の一斉分析

元データ 1985-12-31 社団法人日本薬学会

概要

A high performance liquid chromatographic method (HPLC) was developed for the simultaneous determination of oxytetracycline (OTC), chlortetracycline (CTC), chloramphenicol (CP), sulfamonomethoxine (SMMX), sulfadimethoxine (SDMX), furazolidone (FZ) and difurazone (DFZ) in cultured fish. The drugs were extracted from fish with 0.5% metaphosphoric acid-methanol (8 : 2), and the extract was filtered and concentrated. The concentrated solution was passed through a Sep-pak C_<18> cartridge. The cartridge was washed with water and eluted with methanol. The eluate was evaporated to dryness, and the residue was dissolved in acetonitrile-water (3 : 7). The sample solution was analysed by HPLC using a Nucleosil 5 C_<18> column as a column and acetonitle 0.05 M monobasic sodium phosphate (35 : 65), pH 2.5 as a mobile phase. The average recoveries of OTC, CTC, CP, SMMX, SDMX and FZ from rainbow trouts, yellowtails and red sea breams fortified at 1.0 μg/g were 68.9-91.7%. The limits of detection were 0.05 μg/g for OTC, CP, SMMX, SDMX and FZ, 0.1 μg/g for CTC and 1.0 μg/g for DFZ.

著者

堀江 正一 埼玉県衛生研究所
中澤 裕之 星薬科大学薬品分析化学教室
星野 庸二 埼玉県衛生研究所
堀江 正一 大妻女子大学
能勢 憲英 埼玉県衛生研究所
中澤 裕之 国立公衆衛生院
岩崎 久夫 埼玉県衛生研究所
中澤 裕之 愛知県衛生研究所
岩崎 久夫 埼玉県衛研

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