環境試料中ダイオキシン類の簡易酵素免疫測定法の開発(生命科学と分析化学)
概要
The purpose of this research is the establishment of ELISA for measuring dioxins in environmental samples, such as house dust, soil, exhaust gas and fly ash. Since we have developed dry-type plates in order to put the ELISA system into practical use, the basic performance, such as the cross-reactivity, linearity and the correlation between the conventional GC/MS values and the ELISA values, were studied. The dry plates could be stored for about one year in a refrigerator. Furthermore, a good correlation was observed between the dioxin quantities by ELISA and those by GC/MS in exhaust gas, fly ash, soil and house dust samples. The developed ELISA system indicated usefulness as a toxicity evaluation method for dioxins in environmental samples.